...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Experimental Biology >Targeting of an expressed neurotoxin by its recombinant baculovirus
【24h】

Targeting of an expressed neurotoxin by its recombinant baculovirus

机译：通过重组杆状病毒靶向表达的神经毒素

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AaIT, an insect-selective neurotoxic polypeptide derived from scorpion venom, has recently been used to engineer recombinant baculoviruses for insect pest control. Lepidopterous larvae infected with an AaIT-expressing baculovirus; reveal symptoms of paralysis identical to those induced by injection of the native toxin. However, the paralyzed larvae treated by the recombinant virus possess an approximately 50-fold lower hemolymph toxin concentration than insects paralyzed by the native toxin. The mechanism of this potentiation effect was studied using immunocytochemistry, electrophysiology and toxicity assays. (i) Light microscopy, using peroxidase-conjugated antibodies, revealed the presence of toxin in virus-susceptible tissues, including tracheal epithelia located close to the central nervous system and beyond its lamellar enveloping sheath. (ii) High-resolution immunogold electron microscopical cytochemistry clearly revealed the presence of recombinant AaIT toxin inside the thoracic and abdominal ganglia on neuronal cell bodies and axonal membranes. (iii) Ventral nerve cords dissected from silkworm larvae infected with the recombinant baculovirus exhibited a high degree of excitability, expressed as enhanced frequency and bursting mode of their spontaneous activity, when compared to nerve cords infected with the wild-type virus. We conclude that the recombinant-virus-infected tracheal epithelia, outbranching in the body of an infected insect, (i) locally supply a continuous, freshly produced toxin to its neuronal receptors and (ii) introduce the expressed toxin to the insect central nervous system, thus providing it with critical target sites that are inaccessible to the native toxin.
机译：AaIT是一种源自蝎毒的昆虫选择性神经毒性多肽，最近已用于工程改造重组杆状病毒以控制害虫。鳞翅目幼虫感染了表达AaIT的杆状病毒;揭示出与注射天然毒素诱导的症状相同的瘫痪症状。但是，重组病毒处理过的麻痹幼虫的血淋巴毒素浓度比天然毒素麻痹的昆虫低约50倍。使用免疫细胞化学，电生理学和毒性试验研究了这种增强作用的机制。 （i）使用过氧化物酶偶联抗体的光学显微镜检查显示，在易感病毒的组织中，包括在靠近中枢神经系统且位于其层状包膜鞘之外的气管上皮中，均存在毒素。 （ii）高分辨率免疫金电子显微镜细胞化学清楚地揭示了在神经元细胞体和轴突膜的胸和腹神经节内部存在重组AaIT毒素。 （iii）与感染了野生型病毒的神经线相比，从重组杆状病毒感染的家蚕幼虫解剖的腹侧神经线表现出高度的兴奋性，表现为自发性活动的频率和爆发模式的增强。我们得出的结论是，重组病毒感染的气管上皮在被感染的昆虫体内分支，（i）向其神经元受体局部提供连续的，新鲜产生的毒素，并且（ii）将表达的毒素引入昆虫的中枢神经系统，从而为它提供了天然毒素无法接近的关键目标部位。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号