...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Experimental Biology >Bursting properties of caudal neurosecretory cells in the flounderPlatichthys flesus, in vitro
【24h】

Bursting properties of caudal neurosecretory cells in the flounderPlatichthys flesus, in vitro

机译：比目鱼腓肠尾神经分泌细胞的破裂特性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bursting activity in type 1 Dahlgren cells was studied using intra- and extracellular recording from an in vitro preparation of the caudal neurosecretory system of the euryhaline flounder. 45 % of cells showed spontaneous bursts of approximately 120s duration and 380s cycle period. Similar bursts were triggered by short duration (<5 s) depolarising or hyperpolarising pulses. Cells displayed a characteristic depolarising after potential, following either an action potential with associated afterhyperpolarisation, or a hyperpolarising current pulse. This depolarising after potential was related to a 'sag' potential, which developed during the hyperpolarising pulse. Both the depolarising after potential and the sag potential occurred only in cells at more depolarised (<60 mV) holding potentials. In addition, the amplitude of the depolarising after potential was dependent on the amplitude and the duration of the hyperpolarising pulse. The depolarising after potential following action potentials may provide a mechanism for facilitating repetitive firing during a burst. Extracellular recording revealed similar bursting in individual units which was not, however, synchronised between units. Spontaneous bursting activity recorded both intra- and extracellularly was inhibited by application of a known neuromodulator of the system, 5-hydroxytryptamine. This study provides a basis for investigating the relationship between physiological status, Dahlgren cell activity and neuropeptide secretion.
机译：使用体外制备的鱼腥草比目鱼尾神经分泌系统的细胞内和细胞外记录，研究了1型Dahlgren细胞的爆发活性。 45％的细胞显示约120s持续时间和380s周期的自发爆发。短时间（<5 s）的去极化或超极化脉冲触发了类似的猝发。在具有相关的超极化后的动作电位或超极化电流脉冲之后，细胞在电位后表现出特征性的去极化。电位后的这种去极化与超极化脉冲期间产生的“下陷”电位有关。电位之后的去极化和下陷电位都仅发生在具有更多去极化（<60 mV）保持电位的细胞中。另外，电势后去极化的幅度取决于超极化脉冲的幅度和持续时间。电位跟随动作电位后的去极化可提供促进在猝发期间重复发射的机制。细胞外记录显示单个单元中类似的爆发，但是，单元之间不同步。通过使用系统的已知神经调节剂5-羟色胺抑制了细胞内和细胞外记录的自发爆发活性。该研究为研究生理状态，Dahlgren细胞活性和神经肽分泌之间的关系提供了基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号