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首页> 外文期刊>The Journal of Experimental Biology >Haemolymph sugar levels in foraging honeybees (Apis mellifera carnica):Dependence on metabolic rate and in vivo measurement of maximal rates oftrehalose synthesis
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Haemolymph sugar levels in foraging honeybees (Apis mellifera carnica):Dependence on metabolic rate and in vivo measurement of maximal rates oftrehalose synthesis

机译：觅食蜜蜂的血淋巴糖水平：取决于代谢率和体内海藻糖合成最大速率的测量

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摘要

Previous investigations of haemolymph sugar levels in honeybees have reported very different results, probably because different experimental conditions affected the activity levels of the animals. The present study investigated the dependence of haemolymph sugar levels in foraging honeybees on metabolic rate and whether the haemolymph sugar level is regulated. Free-flying foraging bees were trained to collect controlled amounts of sucrose solution of different concentrations (15 %, 30 % or 50 % sucrose w/w). Immediately after feeding, metabolic rate was measured over a given time depending on the sucrose concentration, then crop-emptying rate and haemolymph sugar levels were measured. Bees exhibiting a wide range of metabolic rates were compared to establish whether the observed differences in haemolymph sugar levels were due to limits in the supply of sugars from the crop or in the rate of trehalose synthesis in the fat bodies. Independent of the concentration of the sucrose solution supplied, haemolymph trehalose, glucose and fructose levels were constant for metabolic rates from 0 to 4.5 ml CO2 h(-1). At higher metabolic rates, trehalose concentration decreased while that of glucose and fructose increased, with the exception of bees fed 15 % sucrose solution. As the supply of sugar from the crop via the proventriculus was sufficient to support even the highest metabolic rates, the observed pattern must result from an upper limit in the capacity of the fat body to synthesise trehalose. The maximal rate of conversion of glucose to trehalose in the fat body was therefore calculated to average 92.4 mug glucose min(-1). However, for bees fed 15 % sucrose solution both the rate of conversion of glucose to trehalose and the rate of sugar transport from the crop to the ventricle were limited, together resulting in a decrease in total haemolymph sugar levels for metabolic rates higher than 5 ml CO2 h(-1).
机译：先前对蜜蜂血淋巴糖水平的调查报告了非常不同的结果，可能是因为不同的实验条件影响了动物的活动水平。本研究调查了觅食蜜蜂中血淋巴糖水平对代谢率的依赖性以及血淋巴糖水平是否受到调节。对自由飞行的觅食蜜蜂进行了培训，以收集可控制量的不同浓度（重量比为15％，30％或50％的蔗糖）的蔗糖溶液。喂食后立即根据蔗糖浓度测量给定时间内的代谢率，然后测量排空率和血淋巴糖水平。比较表现出广泛代谢率的蜜蜂，以确定所观察到的血淋巴糖水平差异是由于农作物中糖的供应受限还是脂肪体内海藻糖合成速率的限制。与所提供的蔗糖溶液浓度无关，血淋巴海藻糖，葡萄糖和果糖水平对于0至4.5 ml CO2 h（-1）的代谢速率是恒定的。在较高的代谢速率下，海藻糖浓度下降，而葡萄糖和果糖的海藻糖浓度增加，但蜜蜂饲喂15％蔗糖溶液除外。由于通过前胚层从作物中供应的糖足以支持甚至最高的代谢速率，因此观察到的模式必须来自脂肪体合成海藻糖能力的上限。因此，计算出脂肪体内葡萄糖向海藻糖的最大转化率平均为92.4马克杯葡萄糖min（-1）。但是，对于以15％蔗糖溶液喂养的蜜蜂，葡萄糖转化为海藻糖的速率以及糖从作物向心室的运输速率都受到限制，导致代谢速率高于5 ml的总血淋巴糖水平降低二氧化碳h（-1）。

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