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首页> 外文期刊>The Journal of Experimental Biology >Mechanisms and costs of mitochondrial thermal acclimation in a eurythermal killifish (Fundulus heteroclitus)
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Mechanisms and costs of mitochondrial thermal acclimation in a eurythermal killifish (Fundulus heteroclitus)

机译：线热thermal鱼（Fundulus heteroclitus）中线粒体热适应的机制和成本

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Processes acting at the level of the mitochondria have been suggested to affect the thermal limits of organisms. To determine whether changes in mitochondrial properties could underlie shifts in thermal limits, we examined how mitochondrial properties are affected by thermal acclimation in the eurythermal killifish, Fundulus heteroclitus - a species with substantial plasticity in whole-organism thermal limits. We hypothesized that thermal acclimation would result in functional changes in the mitochondria that could result in trade-offs in function during acute thermal shifts. We measured the mitochondrial respiration rate ((V)over dot(O2)) through multiple complexes of the electron transport system following thermal acclimation (to 5, 15, 33 degrees C) and assessed maintenance of mitochondrial membrane potential (Delta p) and rates of reactive oxygen species (ROS) production as an estimate of costs. Acclimation to 5 degrees C resulted in a modest compensation of mitochondrial respiration at low temperatures, but these mitochondria were able to maintain Delta p with acute exposure to high temperatures, and ROS production did not differ between acclimation groups, suggesting that these increases in mitochondrial capacity do not alter mitochondrial thermal sensitivity. Acclimation to 33 degrees C suppressed mitochondrial respiration as a result of effects on NADH dehydrogenase (complex I). These high-temperature acclimated fish nonetheless maintained levels of Delta p and ROS production similar to those of the other acclimation groups. This work demonstrates that killifish mitochondria can successfully acclimate to a wide range of temperatures without incurring major functional trade-offs during acute thermal shifts and that high-temperature acclimation results in a suppression of metabolism, consistent with patterns observed at the organismal level.
机译：有人认为作用于线粒体水平的过程会影响生物体的热极限。为了确定线粒体特性的变化是否可能是热极限变化的基础，我们研究了线虫特性如何受到热感kill鱼（Fundulus heteroclitus）中热适应的影响，该物种在整个生物体的热极限中具有可塑性。我们假设热适应会导致线粒体功能发生变化，这可能导致急性热转变期间功能之间的权衡。我们通过热适应（至5、15、33摄氏度）后通过电子传输系统的多个络合物测量线粒体呼吸速率（（V）超过点（O2）），并评估了线粒体膜电位（Δp）和速率的维持活性氧（ROS）生产的成本估算。适应5摄氏度会导致在低温下适度补偿线粒体呼吸，但这些线粒体能够在急性暴露于高温下维持Delta p，并且适应组之间的ROS产量没有差异，表明这些增加了线粒体能力不要改变线粒体的热敏感性。由于对NADH脱氢酶的影响（复合体I），适应33度会抑制线粒体呼吸。但是，这些高温适应鱼的Delta p和ROS产生水平保持与其他适应组相似的水平。这项工作表明，致敏鱼线粒体可以成功适应各种温度，而不会在急性热转变过程中引起重大的功能折衷，并且高温适应导致新陈代谢的抑制，这与在生物体水平上观察到的模式一致。

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