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首页> 外文期刊>The Royal Society Proceedings B: Biological Sciences >Characterization and function of carbonic anhydrases in the zooxanthellae-giant clam symbiosis.
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Characterization and function of carbonic anhydrases in the zooxanthellae-giant clam symbiosis.

机译：虫黄藻-大型蛤共生中碳酸酐酶的特征和功能。

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摘要

Carbonic anhydrase (CA) has been purified from the host tissue of Tridacna gigas, a clam that lives in symbiosis with the dinoflagellate alga, Symbiodinium. At least two isoforms of CA were identified in both gill and mantle tissue. The larger (70 kDa) isoform is a glycoprotein with both N- and O-glycans attached and has highest homology to CAII. It is associated with the membrane fraction while the smaller (32 kDa) is present in the aqueous phase in both tissues. The 32 kDa CA has high homology with mammalian CAI at the N-terminus. Both isoforms cross-reacted with antibodies to CAII from chicken. Immunohistology demonstrated that the 70 kDa CA is present within the ciliated branchial filaments and cells lining the tertiary water channels in the gills of T. gigas. This is consistent with a role in the transport of inorganic carbon (Ci) to the haemolymph and therefore supply of Ci to the zooxanthellae. CA was also detected in mantle epithelial cells where it may also contribute to Ci supply to the zooxanthellae. The hyaline body and nerve tissue in the mantle express the 70 kDa CA where it may be involved in light sensing and nervous transmission.
机译：碳酸酐酶（CA）已从Tridacna gigas的宿主组织中纯化，Tridacna gigas是一种与甲鞭毛藻共生生物共生的蛤。在g和地幔组织中均鉴定出至少两种CA同工型。较大的同工型（70 kDa）是一种糖蛋白，同时附有N和O聚糖，与CAII的同源性最高。它与膜部分有关，而在两个组织的水相中都存在较小的（32 kDa）。 32 kDa CA在N端与哺乳动物CAI具有高度同源性。两种同工型都与来自鸡的CAII抗体发生交叉反应。免疫组织学表明，在巨鳞T的第三级水通道内的纤毛状分支细丝和细胞中存在70 kDa的CA。这与在将无机碳（Ci）转运到血淋巴中以及因此将Ci输送到虫黄藻中的作用一致。在地幔上皮细胞中也检测到了CA，它也可能有助于向黄原虫提供Ci。地幔中的玻璃体和神经组织表达70 kDa CA，可能参与光感测和神经传递。

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