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首页> 外文期刊>The Royal Society Proceedings B: Biological Sciences >Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences.
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Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences.

机译：使用wsp基因序列对Wolbachia菌株进行系统发育和基于PCR的分类。

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摘要

Wolbachia are a group of intracellular inherited bacteria that infect a wide range of arthropods. They are associated with a number of different reproductive phenotypes in their hosts, such as cytoplasmic incompatibility, parthenogenesis and feminization. While it is known that the bacterial strains responsible for these different host phenotypes form a single clade within the alpha-Proteobacteria, until now it has not been possible to resolve the evolutionary relationships between different Wolbachia strains. To address this issue we have cloned and sequenced a gene encoding a surface protein of Wolbachia (wsp) from a representative sample of 28 Wolbachia strains. The sequences from this gene were highly variable and could be used to resolve the phylogenetic relationships of different Wolbachia strains. Based on the sequence of the wsp gene from different Wolbachia isolates we propose that the Wolbachia pipientis clade be initially divided into 12 groups. As more sequence information becomes available we expect the number of such groups to increase. In addition, we present a method of Wolbachia classification based on the use of group-specific wsp polymerase chain reaction (PGR) primers which will allow Wolbachia isolates to be typed without the need to clone and sequence individual Wolbachia genes. This system should facilitate future studies investigating the distribution and biology of Wolbachia strains from large samples of different host species.
机译：沃尔巴氏菌是一组感染多种节肢动物的细胞内遗传细菌。它们与宿主中许多不同的生殖表型有关，例如细胞质不相容，孤雌生殖和女性化。虽然已知负责这些不同宿主表型的细菌菌株在α-Proteobacteria中形成单个进化枝，但到目前为止，尚不可能解决不同Wolbachia菌株之间的进化关系。为了解决这个问题，我们从28个Wolbachia菌株的代表性样本中克隆了一个编码Wolbachia（wsp）表面蛋白的基因并进行了测序。来自该基因的序列是高度可变的，可以用于解决不同的沃尔巴克氏菌菌株的系统发育关系。基于来自不同Wolbachia分离株的wsp基因的序列，我们建议将Wipbachia pipientis进化枝最初分为12组。随着更多的序列信息变得可用，我们期望此类组的数量会增加。此外，我们基于组特异性wsp聚合酶链反应（PGR）引物的使用，提出了一种Wolbachia分类的方法，该引物将允许对Wolbachia分离株进行分型，而无需克隆和测序单个Wolbachia基因。该系统应有助于未来的研究，以调查来自不同宿主物种的大量样品中的沃尔巴克氏菌菌株的分布和生物学。

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