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首页> 外文期刊>The Plant Cell >A Heterocomplex of Iron Superoxide Dismutases Defends Chloroplast Nucleoids against Oxidative Stress and Is Essential for Chloroplast Development in Arabidopsis
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A Heterocomplex of Iron Superoxide Dismutases Defends Chloroplast Nucleoids against Oxidative Stress and Is Essential for Chloroplast Development in Arabidopsis

机译：铁超氧化物歧化酶的杂合物捍卫叶绿体核抗氧化应激，是拟南芥叶绿体发育必不可少的。

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摘要

There are three iron superoxide dismutases in Arabidopsis thaliana: FE SUPEROXIDE DISMUTASE1 (FSD1), FSD2, and FSD3. Their biological roles in chloroplast development are unknown. Here, we show that FSD2 and FSD3 play essential roles in early chloroplast development, whereas FSD1, which is found in the cytoplasm, does not. An fsd2-1 fsd3-1 double mutant had a severe albino phenotype on agar plates, whereas fsd2 and fsd3 single knockout mutants had pale green phenotypes. Chloroplast development was arrested in young seedlings of the double mutant. The mutant plants were highly sensitive to oxidative stress and developed increased levels of reactive oxygen species (ROS) during extended darkness. The FSD2 and FSD3 proteins formed a heteromeric protein complex in the chloroplast nucleoids. Furthermore, transgenic Arabidopsis plants overexpressing both the FSD2 and FSD3 genes showed greater tolerance to oxidative stress induced by methyl viologen than did the wild type or single FSD2- or FSD3-overexpressing lines. We propose that heteromeric FSD2 and FSD3 act as ROS scavengers in the maintenance of early chloroplast development by protecting the chloroplast nucleoids from ROS.
机译：拟南芥中存在三种铁超氧化物歧化酶：FE SUPEROXIDE DISMUTASE1（FSD1），FSD2和FSD3。它们在叶绿体发育中的生物学作用尚不清楚。在这里，我们显示FSD2和FSD3在早期叶绿体发育中起重要作用，而在细胞质中发现的FSD1则没有。 fsd2-1 fsd3-1双突变体在琼脂平板上具有严重的白化病表型，而fsd2和fsd3单敲除突变体具有淡绿色表型。叶绿体发育被阻止在双突变体的幼苗中。突变植物对氧化应激高度敏感，并在延长的黑暗中增加了活性氧（ROS）的水平。 FSD2和FSD3蛋白在叶绿体核苷酸中形成了异源蛋白复合物。此外，与野生型或单个FSD2或FSD3过表达系相比，过表达FSD2和FSD3基因的转基因拟南芥植物对甲基紫精诱导的氧化胁迫表现出更大的耐受性。我们提出，通过保护叶绿体核苷酸免受ROS的影响，异聚FSD2和FSD3在维持早期叶绿体发育中充当ROS清除剂。

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