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首页> 外文期刊>The Southeast Asian journal of tropical medicine and public health >Development of a comprehensive red blood cell enzymopathy laboratory in Thailand: the study of normal activity in eight erythroenzymes in Thais.
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Development of a comprehensive red blood cell enzymopathy laboratory in Thailand: the study of normal activity in eight erythroenzymes in Thais.

机译：在泰国建立一个综合性红细胞酶病实验室：在泰国人中研究八种红酶的正常活性。

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摘要

In order to provide a reference range for normal red blood cell enzyme activities in Thai, we analyzed data from 113 healthy non-anemic Thai people (55 males and 58 females) age 1-42 years, who all had a normal pattern of hemoglobin typing (HbA and HbA2 less than 3.5%). Hematological analysis was performed using an automated cell counter and the hemoglobin studies were carried out by low pressure liquid chromatography. Owing to a high frequency of alpha-thalassemia in Thailand, cases with an MCV < 75 fl were excluded from the study since these cases were likely to be heterozygotes for alpha0-thalassemia. Cases with reticulocytes > 2.5% were excluded from the study since reticulocytes have a higher enzyme activity than mature erythrocytes. Cases with abnormal red blood cell morphology, such as spherocytes and ovalocytes, were also excluded. These criteria were applied to select "normal" controls for our analysis. We assayed eight red blood cell enzyme activities in normal subjects: glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), pyruvate kinase (PK), hexokinase (HK), glucose phosphate isomerase (GPI), phosphofructokinase (PFK), aldolase (ALD) and phosphoglycerate kinase (PGK). The mean normal ranges (+/- SD) for G6PD, 6PGD, PK, HK, GPI, PFK, ALD and PGK were 12.7 (+/-2.2), 10.7 (+/-1.3), 18.5 (+/-4.0), 1.5 (+/-0.4), 80.5 (+/-11.8), 11.8 (+/-2.1), 4.5 (+/-1.6) and 370 (+/-43) IU/gHb, respectively. Age-dependent differences for the reference values for these enzyme activities were summarized. All red blood cell enzyme activities were highest during the early childhood period and slightly lower in the adult period. These values will be of clinically useful for future reference.
机译：为了为泰国人的正常红细胞酶活性提供参考范围，我们分析了来自113名年龄在1-42岁的健康非贫血泰国人（男性55岁，女性58岁）的数据，这些人均具有正常的血红蛋白分型（HbA和HbA2小于3.5％）。使用自动细胞计数器进行血液学分析，并通过低压液相色谱法进行血红蛋白研究。由于泰国的α地中海贫血发生率很高，因此将MCV <75 fl的病例排除在研究之外，因为这些病例很可能是α0地中海贫血的杂合子。网织红细胞> 2.5％的病例被排除在研究之外，因为网织红细胞比成熟红细胞具有更高的酶活性。还排除了具有异常红细胞形态的病例，如球细胞和卵母细胞。这些标准用于选择“正常”对照进行分析。我们在正常受试者中检测了八种红细胞酶活性：6-磷酸葡萄糖脱氢酶（G6PD），6-磷酸葡萄糖酸酯脱氢酶（6PGD），丙酮酸激酶（PK），己糖激酶（HK），葡萄糖磷酸异构酶（GPI），磷酸果糖激酶（ PFK），醛缩酶（ALD）和磷酸甘油酸激酶（PGK）。 G6PD，6PGD，PK，HK，GPI，PFK，ALD和PGK的平均正常范围（+/- SD）为12.7（+/- 2.2），10.7（+/- 1.3），18.5（+/- 4.0）分别为1.5（+/- 0.4），80.5（+/- 11.8），11.8（+/- 2.1），4.5（+/- 1.6）和370（+/- 43）IU / gHb。总结了这些酶活性参考值的年龄依赖性差异。在幼儿期，所有的红细胞酶活性最高，而在成年期则略低。这些值将在临床上有用，以备将来参考。

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