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首页> 外文期刊>The Southeast Asian journal of tropical medicine and public health >LOCALIZATION OF THE CYSTOGENOUS GLANDS OF OPISTHORCHIS VIVERRINI CERCARIAE
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LOCALIZATION OF THE CYSTOGENOUS GLANDS OF OPISTHORCHIS VIVERRINI CERCARIAE

机译：阿维氏虾IST胞内腺的定位。

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摘要

Opisthorchiasis in northeastern Thailand is an important etiology of cholangiocarcinoma. To form the infectious stage, free swimming cercariae penetrate cyprinid fish, shed their tails, and then secret a cystic substance to cover their larval stage to form metacercariae in the fish body. We determined the location of the cystogenous glands in Opisthorchis viverrini cercariae. The cercariae and metacercariae were obtained from the naturally infected snail host, Bithynia siamensis goniomphalos and from cyprinid fish, respectively. The cyst walls of the metacercariae were separated and used to immunize inbred male BALB/c mice to obtain cyst wall antibodies. The general characteristics of the O. viverrini cercariae and metacercariae were studied by hematoxylin and eosin (H&E) staining of sections. The location and ultrastructure of the cystogenous glands of cercariae were studied by immunoperoxidase, immunofluorescence and transmission electron microscopy. The structures and organelles of cercariae and metacercariae could be identified, but the cystogenous glands could not be detected in H&E sections. The immunoperoxidase and immunofluorescence sections revealed positive reactions for cystogenous glands predominanted in the lateral part of the cercariae and were clearly seen in the cyst wall of the metacercariae. The ultrastructure of the cystogenous glands contained semitranslucent electron dense oval shaped granules. If interference occurs during the formation of the cysts by fish immune response, the metacercariae may not develop to maturity. It may be easily digested or degraded by human stomach acid and pepsin. This may be an efficient method for control of O. viverrini infection which requires further detailed study.
机译：泰国东北部的羊膜毛虫病是胆管癌的重要病因。为了形成感染阶段，自由游泳的尾c会穿透塞浦路斯鱼类，掉下尾巴，然后分泌一种囊性物质以覆盖其幼虫期，从而在鱼体内形成meta尾虫。我们确定了Opisthorchis viverrini cercariae中囊性腺的位置。尾c和meta尾aria分别得自自然感染的蜗牛寄主Bithynia siamensis goniomphalos和pr鱼。分离尾cer的囊壁并用于免疫近交雄性BALB / c小鼠以获得囊壁抗体。通过苏木精和曙红（H＆E）染色切片研究了维氏弧菌尾meta和aria尾的一般特征。通过免疫过氧化物酶，免疫荧光和透射电镜研究了尾c囊状腺的位置和超微结构。可以确定尾c和尾cer的结构和细胞器，但在H＆E切片中未检测到囊性腺。免疫过氧化物酶和免疫荧光切片显示，在尾c的外侧部分主要存在的囊性腺阳性反应，并且在meta尾的囊壁清楚可见。囊性腺体的超微结构包含半透明的电子致密椭圆形颗粒。如果通过鱼的免疫反应在囊肿形成过程中发生干扰，则cer尾aria可能不会发育成熟。它很容易被人的胃酸和胃蛋白酶消化或降解。这可能是控制维氏弧菌感染的有效方法，需要进一步详细研究。

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