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首页> 外文期刊>The Southeast Asian journal of tropical medicine and public health >GENOTYPE AND DRUG RESISTANCE OF CLINICAL AND ENVIRONMENTAL VIBRIO CHOLERAE NON-O1/NON-O139 IN NORTHEASTERN THAILAND
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GENOTYPE AND DRUG RESISTANCE OF CLINICAL AND ENVIRONMENTAL VIBRIO CHOLERAE NON-O1/NON-O139 IN NORTHEASTERN THAILAND

机译：泰国东北部非O1 /非O139霍乱弧菌的基因型和药物耐药性

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A total of 124 V. cholerae non-O1on-O139 isolates were collected in Khon Kaen, Thailand from diarrheal patients, asymptomatic carriers and environmental water. The presence of virulence-associated and regulatory genes including ctxA, tcpA, zot, ace, ompU, stn, hlyA and toxR) were examined using multiplex PCR. The genomic diversity of the various V cholerae isolates were differentiated using the random amplified polymorphic DNA (RAPD) method. Antimicrobial susceptibility was tested using disk diffusion. All of V. cholerae non-O1on-O139 isolates carried hlyA and toxR and none carried ctxA and tcpA. The zot, ace and both genes together were found in 1.6%, 4.7% and 4.7% of 64 clinical V. cholerae non-O1 isolates, respectively, while the environmental ones did not. The stn gene was found in 3.1% (2/64) of the clinical and 3.3% (2/60) of the environmental isolates. The RAPD patterns were differentiated into 45 types (A to 2S). RAPD type A (32.3%) was the most frequently found in both clinical and environmental V. cholerae non-O1 strains (34.4% and 30.0%, respectively); indicating that there was a clonal relationship between some clinical and environmental isolates whereas almost all of the environmental isolates belonged to different clones. All strains were sensitive to ciprofloxacin and norfloxacin. The environmental isolates (30%) were more resistant than the clinical ones (21.9%). Resistance to sulfamethoxazole / trimethoprim and tetracycline among the clinical isolates occurred in 9.4% (6/64) in 2007, during which period the prevalence of V. cholerae 01 increased. We conclude that V. cholerae non-O1on-O139 from the aquatic environment are potentially pathogenic and this same aquatic environment may be a source of antimicrobial resistance in V. cholerae.
机译：在泰国孔敬市，从腹泻患者，无症状携带者和环境水中收集了总共124株霍乱弧菌非O1 /非O139分离株。使用多重PCR检查了毒力相关基因和调控基因，包括ctxA，tcpA，zot，ace，ompU，stn，hlyA和toxR）的存在。使用随机扩增多态性DNA（RAPD）方法区分了各种霍乱弧菌的基因组多样性。使用圆盘扩散法测试了抗生素敏感性。霍乱弧菌的所有非O1 /非O139分离株均携带hlyA和toxR，均不携带ctxA和tcpA。在64例临床霍乱弧菌非O1分离株中，分别发现了zot，ace和这两个基因，分别为1.6％，4.7％和4.7％，而环境中则没有。在临床分离株的3.1％（2/64）和环境分离株的3.3％（2/60）中发现了stn基因。 RAPD模式分为45种类型（A至2S）。在临床和环境霍乱弧菌非O1菌株中，RAPD A型（占32.3％）是最常见的（分别为34.4％和30.0％）。这表明某些临床分离株与环境分离株之间存在克隆关系，而几乎所有环境分离株都属于不同的克隆。所有菌株均对环丙沙星和诺氟沙星敏感。环境分离株（30％）比临床分离株（21.9％）更有抵抗力。 2007年临床分离株中对磺胺甲恶唑/甲氧苄啶和四环素的耐药性占9.4％（6/64），在此期间霍乱弧菌01的患病率增加。我们得出的结论是，水生环境中的霍乱弧菌非O1 / non-O139可能具有致病性，而同一水生环境可能是霍乱弧菌抗药性的来源。

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