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首页> 外文期刊>The Southeast Asian journal of tropical medicine and public health >GENETIC SUBTYPES OF BLASTOCYSTIS ISOLATED FROM THAI HOSPITALIZED PATIENTS IN NORTHEASTERN THAILAND
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GENETIC SUBTYPES OF BLASTOCYSTIS ISOLATED FROM THAI HOSPITALIZED PATIENTS IN NORTHEASTERN THAILAND

机译：东北泰国泰式住院患者分离出的胚性败血病的遗传亚型

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摘要

Blastocystis sp is probably the most common intestinal protozoan of humans. This taxon is known to include more than 17 subtypes, some of which likely cause human disease. We investigated the distribution of Blastocystis subtypes in Thai patients admitted for a variety of conditions at a hospital in northeastern Thailand. Fresh fecal samples, positive for Blastocystis by microscopy, were individually cultured in Jones' medium (n = 20) and each sample was used for amplification and sequencing a fragment of 18S rDNA. BLAST search and phylogenetic analysis demonstrated that Blastocystis subtypes ST1 (20%), ST3 (60%), ST6 (10%) and ST7 (10%) were present. No clear link between gastro-intestinal symptoms and any particular subtype of Blastocystis was apparent. Thus, there is a need to extend the work to evaluate clinical signs and subtypes in a larger cohort of patients.
机译：胚芽孢杆菌可能是人类最常见的肠道原生动物。已知该分类单元包括17种以上的亚型，其中一些可能引起人类疾病。我们调查了泰国东北部一家医院因各种情况而入院的泰国患者中的囊胚亚型分布。在琼斯培养基（n = 20）中分别培养新鲜的粪便样本（通过显微镜检对囊胚呈阳性），并将每个样本用于18S rDNA片段的扩增和测序。 BLAST搜索和系统发育分析表明，存在囊胚亚型ST1（20％），ST3（60％），ST6（10％）和ST7（10％）。胃肠道症状与任何特定的Blastocystis亚型之间没有明确的联系。因此，有必要将工作扩展到评估更多患者的临床体征和亚型。

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