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首页> 外文期刊>The Southeast Asian journal of tropical medicine and public health >IMMUNO-MAGNETIC ISOLATION, CHARACTERIZATION AND GENETIC RELATIONSHIP OF ESCHERICHIA COLI O26 FROM RAW MEATS, HAT YAI CITY, SONGKHLA, THAILAND
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IMMUNO-MAGNETIC ISOLATION, CHARACTERIZATION AND GENETIC RELATIONSHIP OF ESCHERICHIA COLI O26 FROM RAW MEATS, HAT YAI CITY, SONGKHLA, THAILAND

机译：泰国宋卡府合艾原产大肠埃希氏菌O26的免疫磁分离，表征和遗传关系

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摘要

Escherichia coil O26 is the most important serotype in non-O157 group, which plays a significant role in gastrointestinal illnesses. However, information regarding the prevalence and its characteristics are lacking in Thailand. As raw meat is frequently a source of diarrheagenic E. coli, a total of 1,279 E. coli colonies were obtained from 157 raw meat samples obtained from retail markets in Hat Yai City, Songkhla Province, Thailand and E. coil O26 isolated using an immunemagnetic separation technique. Twenty-seven E. coil O26 strains were isolated from 18 samples of raw beef, chicken and pork meats. These E. coil O26 strains could not be classified into the six diarrheagenic E. coil categories and did not harbor virulence genes, except 5 strains carrying escV, encoding type III secretion system component. Phylogenetic group examination demonstrated that 26 strains belonged to phylogenetic group A, and one to group D. Antimicrobial susceptibility test revealed that the E. coli O26 strains were the multi-drug resistant strains. Genetic relatedness employing (GTG)(5)-PCR and ERIC2-PCR showed that some of O26 which isolated from different samples and different time intervals revealed the identical fingerprint pattern, suggesting that they were derived from the same clone. Examination of five stx(2)-containing phage integration sites showed that 6 strains had prophage occupancy at sbcB, suggesting that these isolates have the potential in horizontal gene transfer of virulence trait. Moreover, the intactness of yecE and wrbA, the important integration sites in E. coil O26, indicated the possibility of stx(2)-phage lysogenization in the future.
机译：大肠杆菌O26是非O157组中最重要的血清型，在胃肠道疾病中起重要作用。但是，泰国缺乏有关流行及其特征的信息。由于生肉经常是引起腹泻的大肠杆菌的来源，因此从泰国宋卡府合艾市的零售市场获得的157个生肉样品中获得了1,279个大肠杆菌菌落，并使用免疫磁法分离了O26大肠杆菌。分离技术。从18个生牛肉，鸡肉和猪肉样品中分离出27个大肠杆菌O26菌株。除了5株携带escV编码III型分泌系统成分的菌株外，这些O.coil O26菌株无法分类为6个腹泻性E.coil菌株，并且没有携带毒力基因。系统进化组检查表明，有26个菌株属于系统发育A组，其中一个属于D组。抗药性试验表明，大肠杆菌O26菌株是多药耐药菌株。利用（GTG）（5）-PCR和ERIC2-PCR进行的遗传相关性分析表明，从不同样品和不同时间间隔分离出的O26中的某些指纹图谱具有相同的指纹图谱，表明它们来源于同一克隆。五个stx（2）包含噬菌体整合位点的检查表明，有6个菌株在sbcB上有噬菌体占有，这表明这些分离株具有潜在的水平基因转移能力。此外，yecE和wrbA（大肠杆菌O26的重要整合位点）的完整度表明将来可能发生stx（2）噬菌体裂解。

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