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首页> 外文期刊>The spine journal: official journal of the North American Spine Society >Comparative study of fusion rate induced by different dosages of Escherichia coli-derived recombinant human bone morphogenetic protein-2 using hydroxyapatite carrier
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Comparative study of fusion rate induced by different dosages of Escherichia coli-derived recombinant human bone morphogenetic protein-2 using hydroxyapatite carrier

机译：羟基磷灰石载体不同剂量大肠杆菌重组人骨形态发生蛋白2诱导融合速率的比较研究

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Background context: Hydroxyapatite (HA) is considered to be useful because of its high affinity for recombinant human bone morphogenetic protein (rhBMP), mechanical resistance to compressive force, and possible reduction of rhBMP dose. Purpose: To evaluate the osteoinductivity of Escherichia coli-derived rhBMP-2 and the suitability of porous HA as an rhBMP-2 carrier. Study design: In vivo study using microcomputerized tomography (micro-CT) scanning. Patient sample: Seventy-six New Zealand white male rabbits were randomized into a single control group (n=14) without rhBMP-2 and four experimental groups (10 μg, 50 μg, 200 μg, and 500 μg of rhBMP-2; n=14 in each group). The subjects were divided into 3- and 6-week groups. Outcome measures: Outcome was evaluated by radiography, bending test, three-dimensional micro-CT, and histologic examinations. Methods: Bilateral posterolateral fusion was carried out, and rhBMP-2 (0, 10, 50, 200, 500, 1,000, and 2,000 μg) was implanted into the bilateral transverse processes using HA as a carrier. Results: The fusion rates of the 3-week group were 83.3% for 50 and 200 μg of rhBMP-2 and 100% for 500 μg. The improved fusion rates of the 50 μg or higher groups compared with those of control were statistically significant. The fusion rates of the 6-week group were 75% for 10 μg of rhBMP-2 and 100% for 50 μg or higher. Similarly, the improved fusion rates of the 10 μg or higher groups compared with those of control were statistically significant. Significantly higher percent volumes were observed in the 3-week 200 μg of rhBMP-2 group and 6-week 200 μg of rhBMP-2 group than the 3-week HA group and 6-week HA group, respectively. Trabecular thickness was significantly higher in the 3-week 200 μg of rhBMP-2 group than the 3-week HA group. Histologic analysis of the 10 μg group showed bone tissues within the pores from 3 weeks, and this was observed more vividly in the 50, 200, and 500 μg groups. The 6-week 10 μg and 50 μg of rhBMP-2 groups had lower amounts of new tissue but higher portions of complete bone tissue within the HA specimen, along with higher formation of completely reconstituted bone tissues outside HA. Conclusions: Injection of 50 μg or more of E. coli-derived rhBMP-2 into a HA carrier induced earlier bone fusion in the intertransverse process of rabbits, which confirms the excellent bone forming ability of E. coli-derived rhBMP-2 and the suitability of HA as a carrier of rhBMP-2.
机译：背景技术：羟基磷灰石（HA）被认为是有用的，因为它对重组人骨形态发生蛋白（rhBMP）具有高亲和力，对压缩力的机械抗性以及可能降低rhBMP剂量。目的：评价大肠杆菌衍生的rhBMP-2的骨诱导性，以及多孔HA作为rhBMP-2载体的适用性。研究设计：使用微计算机断层扫描（micro-CT）扫描的体内研究。患者样品：将76只新西兰白雄兔随机分为不包含rhBMP-2的单个对照组（n = 14）和四个实验组（10μg，50μg，200μg和500μgrhBMP-2; n每组= 14）。将受试者分为3周和6周组。结果测量：通过射线照相，弯曲试验，三维显微CT和组织学检查对结果进行评估。方法：进行双侧后外侧融合术，并以HA为载体将rhBMP-2（0、10、50、200、500、1,000和2,000μg）植入双侧横突。结果：3周组的融合率分别为50和200μgrhBMP-2为83.3％，而500μg为100％。与对照组相比，50μg或更高组的融合率有统计学意义。 6周组的融合率对于10μgrhBMP-2为75％，对于50μg或更高为100％。同样，与对照组相比，10μg或更高组的融合率有统计学意义。在3周的200μgrhBMP-2组和6周的200μgrhBMP-2组中，观察到的体积百分比分别明显高于3周的HA组和6周的HA组。 3周200μgrhBMP-2组的小梁厚度明显高于3周HA组。 10μg组的组织学分析显示3周后毛孔内有骨组织，在50、200和500μg组中观察到更清晰。 6周的10μg和50μgrhBMP-2组在HA标本中的新组织数量较少，但完整骨组织的含量较高，而在HA外部完全重建的骨组织的形成较高。结论：将50μg或更多的大肠杆菌来源的rhBMP-2注射到HA载体中，可在兔的横断过程中早期融合骨，这证实了大肠杆菌来源的rhBMP-2和其良好的成骨能力。 HA作为rhBMP-2载体的适用性。

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