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首页> 外文期刊>The Pediatric infectious disease journal >Viral etiology of acute respiratory tract infections in children presenting to hospital: role of polymerase chain reaction and demonstration of multiple infections.
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Viral etiology of acute respiratory tract infections in children presenting to hospital: role of polymerase chain reaction and demonstration of multiple infections.

机译：就诊儿童急性呼吸道感染的病毒病因：聚合酶链反应的作用和多种感染的表现。

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摘要

BACKGROUND: Viral lower respiratory tract infections are a leading cause of hospitalization for young children. METHODS: We used polymerase chain reaction (PCR) and conventional methods of cell culture and antigen detection to establish the viral etiology of acute respiratory tract infections in 75 hospitalized children. RESULTS: One or more viral pathogens were detected in 65 (87%) children, with respiratory syncytial virus being the most commonly identified virus (36 children). Other viruses identified included influenza virus types A and B, parainfluenzavirus type 3, adenovirus, enterovirus, rhinovirus, coronavirus and human metapneumovirus. PCR increased the diagnostic yield significantly compared with antigen detection and culture, with 39 (21%) diagnoses identified by this method. Multiple infections were identified in 20 (27%) children. CONCLUSIONS: PCR-based methodologies offer increased sensitivity for the detection of most respiratory viruses in young children. The inclusion of PCR into diagnostic testing strategies is needed to broaden our understanding of the natural ecology of respiratory viruses and the significance of multiple infections.
机译：背景：病毒性下呼吸道感染是幼儿住院的主要原因。方法：我们使用聚合酶链反应（PCR）以及常规的细胞培养和抗原检测方法，建立了75名住院儿童的急性呼吸道感染的病毒病因。结果：在65名（87％）儿童中检测到一种或多种病毒病原体，其中呼吸道合胞病毒是最常见的病毒（36名儿童）。确定的其他病毒包括A型和B型流感病毒，3型副流感病毒，腺病毒，肠病毒，鼻病毒，冠状病毒和人间质肺病毒。与抗原检测和培养相比，PCR可显着提高诊断产量，其中39种（21％）诊断可通过这种方法进行。在20名（27％）儿童中发现了多种感染。结论：基于PCR的方法为检测幼儿中大多数呼吸道病毒提供了更高的灵敏度。需要将PCR纳入诊断测试策略，以加深我们对呼吸道病毒的自然生态学和多重感染的重要性的理解。

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