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IMPROVED LABORATORY DIAGNOSIS OF OVINE FOOTROT - AN UPDATE

机译:改进的卵英尺实验室诊断-最新进展。

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摘要

Ovine footrot is a complex clinical disease syndrome primarily resulting from infection by the anaerobic bacterium Dichelobacter nodosus. In order to aid clinical diagnosis, various laboratory tests based on the detection and measurements of phenotypic properties of D. nodosus have been developed for genus-specific detection as well as virulence determination. However, these tests are generally time-consuming, and tend to be variable with external factors that affect the growth and metabolism of the bacterium. A new generation of diagnostic reagents, such as monoclonal antibodies, gene probes and polymerase chain reaction, has been developed recently. Preliminary assessment of these reagents has shown potential to vastly improve the laboratory identification and determination of the virulence of D. nodosus. It is important that these ne iv reagents are vigorously assessed against existing laboratory tests, such as the elastase test and gelatin gel test, prior to their adoption for the routine diagnosis of footrot.
机译:羊脚腐病是一种复杂的临床疾病综合症,主要是由厌氧细菌结节杆菌感染引起的。为了帮助临床诊断,已经开发了基于对结节藻表型特性的检测和测量的各种实验室测试来进行属特异性检测和毒力测定。但是,这些测试通常很耗时,并且往往会因影响细菌生长和代谢的外部因素而变化。最近已经开发了新一代的诊断试剂,例如单克隆抗体,基因探针和聚合酶链反应。对这些试剂的初步评估已显示出潜力,可以极大地改善实验室鉴定和结节杆菌毒力的测定。重要的是,在将这些新试剂用于常规诊断脚癣之前,必须对现有试剂进行严格评估,例如弹性蛋白酶测试和明胶凝胶测试。

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