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首页> 外文期刊>The Veterinary Journal >The flow cytometric evaluation of phagocytosis by equine peripheral bloodneutrophils and pulmonary alveolar macrophages
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The flow cytometric evaluation of phagocytosis by equine peripheral bloodneutrophils and pulmonary alveolar macrophages

机译：马外周血中性粒细胞和肺泡巨噬细胞对吞噬作用的流式细胞术评估

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摘要

Flow cytometry was used to assess the phagocytosis of fluorescent-labelled bacteria hy equine peripheral blood neutrophils and pulmonary alveolar macrophages. Cell populations were prepared from venous blood following ammonium chloride lysis and from washed bronchoalveolar lavage derived samples. Discrete clusters of cells, corresponding to different leucocyte groups, were readily identified on the basis of differing light scattering properties and could thus be discriminated, negating the need for prior cell separation. Cells able to associate with fluorescent-labelled bacteria (by attachment to the cell membrane or by internalization within the cell) acquired increased fluorescence and were readily differentiated from cells unable to interact with bacteria. The fluorescence of bacteria attached to the cell surface was quenched by the addition of tri pall blue or counterstained by the addition of ethidium bromide to the assay, thus permitting identification of cells which were able to internalize bacteria.
机译：流式细胞仪用于评估马外周血中性粒细胞和肺泡巨噬细胞中荧光标记细菌的吞噬作用。从氯化铵裂解后的静脉血和洗过的支气管肺泡灌洗液衍生的样品中制备细胞群。根据不同的光散射特性，可以容易地识别出与不同白细胞群相对应的离散细胞簇，因此可以进行区分，从而无需进行先前的细胞分离。能够与荧光标记细菌结合的细胞（通过附着在细胞膜上或通过细胞内在化）获得增强的荧光，并容易与无法与细菌相互作用的细胞区分开。通过添加三重苍白猝灭附着在细胞表面的细菌的荧光，或通过在测定中加入溴化乙锭对它进行染色，从而可以鉴定能够使细菌内化的细胞。

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