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A comparison of methods used in species identification ofcoagulase-negative staphylococci isolated from the milk of sheep

机译:绵羊乳中分离的凝固酶阴性葡萄球菌种类鉴定方法的比较

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摘要

Thirty reference strains of staphylococci, 76 strains isolated from confirmed cases of subclinical mastitis and 10 strains from the teat tip skin and the mouth of sucking lambs were assigned to species using four biochemical methods. These were the commercially available micromethods API Staph and Staph-Zym; the specialized laboratory method Rosco Set and Reactions in Standard Laboratory Culture Media (SLCM). The Rosco Set assigned species to all the strains, the SLCM to 96.5%, the Staph-Zym to 86.1% and the API Staph to 81.4%. API Staph and the SLCM favoured the 'aureus' group with the 'epidermidis' second, while the reverse was true for the other two methods. All the identification methods assigned the same species to only 29% of the tested isolates, causing considerable variation in the number of species identified within each group. This variation indicates that reported species prevalence from cases of ovine mastitis should be viewed with caution.
机译:使用四种生化方法将30株葡萄球菌参考菌株,从确诊的亚临床乳腺炎病例中分离出的76株,从乳头尖端皮肤和吮吸羔羊口中分离出的10株进行了分类。这些是可商购的微方法API Staph和Staph-Zym;标准实验室培养基(SLCM)中的专用实验室方法Rosco固定和反应。 Rosco Set为所有菌株指定了物种,SLCM为96.5%,Staph-Zym为86.1%,API Staph为81.4%。 API Staph和SLCM以“金黄色葡萄球菌”(Eureus)组名列第二,而以“表皮”作为第二名,而其他两种方法则相反。所有的鉴定方法只将相同物种分配给29%的测试分离株,从而导致每组中鉴定出的物种数量发生很大变化。这种差异表明,应谨慎对待所报告的绵羊乳腺炎病例的流行率。

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