Isolation and identification of canine matrix metalloproteinase-2 (MMP-2)

Coughlan AR.; Robertson DHL.; Burke R.; Beynon RJ.; Carter SD.

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Isolation and identification of canine matrix metalloproteinase-2 (MMP-2)

机译：犬基质金属蛋白酶-2（MMP-2）的分离与鉴定

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A canine gelatinase, with an apparent molecular mass of 62kDa in non-reducing zymography, is produced by fibroblasts, chondrocytes and a myelomonocytic cell line. The enzyme has similar characteristics to human matrix metalloproteinase (MMP) 2 and cross-reacts in Western blotting analysis with a sheep polyclonal antiserum raised against human MMP-2. The 62kDa canine protein was purified from cell culture media, and the N-terminal amino acid sequence determined following blotting on to a polyvinylidene difluoride (PVDF) membrane. The sequence was 87% identical to that published for human MMP-2. We therefore consider this enzyme to be canine MMP-2.

机译：由成纤维细胞，软骨细胞和骨髓单核细胞系产生的犬明胶酶在非还原酶谱中的表观分子量为62kDa。该酶具有与人基质金属蛋白酶（MMP）2相似的特性，并且在Western blotting分析中与针对人MMP-2的绵羊多克隆抗血清产生交叉反应。从细胞培养基中纯化出62kDa的犬蛋白，印迹到聚偏二氟乙烯（PVDF）膜上后确定N端氨基酸序列。该序列与人MMP-2公开的序列87％相同。因此，我们认为该酶是犬MMP-2。

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《The Veterinary Journal》 |1998年第3期|共7页
作者
Coughlan AR.; Robertson DHL.; Burke R.; Beynon RJ.; Carter SD.;
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正文语种 eng
中图分类动物医学（兽医学）;
关键词
Mmp; Gelatinase; Dog; Purification; Sequencing; Tissue inhibitor; Tumor-cells; Progelatinase; Collagenase; Gelatinase; Activation; Membranes; Secrete;

机译：Mmp;明胶酶;狗;纯化;测序;组织抑制剂;肿瘤细胞;前明胶酶;胶原酶;明胶酶;激活;膜;保密;

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1. Isolation and identification of canine matrix metalloproteinase-2 (MMP-2) [J] . Coughlan AR., Robertson DHL., Burke R., The Veterinary Journal . 1998,第3期

机译：犬基质金属蛋白酶-2（MMP-2）的分离与鉴定
2. Localization and quantification of mRNA for matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in human benign and malignant prostatic tissue. [J] . Still K, Robson CN, Autzen P, The Prostate . 2000,第1期

机译：人体良性和恶性前列腺组织中基质金属蛋白酶2（MMP-2）和基质金属蛋白酶2组织抑制剂（TIMP-2）的mRNA的定位和定量。
3. Identification of activated matrix metalloproteinase-2 (MMP-2) as the main gelatinolytic enzyme in malignant melanoma by in situ zymography. [J] . Kurschat P, Wickenhauser C, Groth W, Journal of Pathology: Journal of the Pathological Society of Great Britain and Ireland . 2002,第2期

机译：通过原位酶谱法鉴定活化基质金属蛋白酶2（MMP-2）是恶性黑色素瘤中主要的明胶分解酶。
4. Targeted Delivery of Peptide-Based Matrix Metalloproteinase-2 (MMP-2) Inhibitor in Dual Sensitive Nanoparticles with Platelet Membrane Camouflage for Ischemic Limb Treatment [C] . Yu Dang, Ning Gao, Hong Niu, Society for Biomaterials annual meeting and exposition . 2019

机译：有针对性的基于肽的基质金属蛋白酶2（MMP-2）抑制剂在带有血小板膜伪装的双敏感纳米颗粒中治疗缺血性肢体的治疗
5. MATRIX-ISOLATION OF OXYGENATED SPECIES FROM PHOTOLYSES OF FORMALDEHYDE, GLYOXAL, HYDROGEN-SULFIDE, CARBONYL-SULFIDE, AND CARBON-DISULFIDE IN AN OXYGEN MATRIX AT 12-19 K THROUGH THE MECHANISM OF EXPANDING PHOTOLYSIS CAGE (MATRIX-ISOLATION, PHOTOOXIDATION). [D] . TSO, TAI-LY. 1984

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6. Identification of collagen binding domain residues that govern catalytic activities of matrix metalloproteinase-2 (MMP-2) [O] . Margarita Mikhailova, Xiaoping Xu, Trista K. Robichaud, -1

机译：鉴定基质金属蛋白酶-2（MMP-2）的催化活性的胶原结合结构域残基
7. Activation of matrix metalloproteinase-2 (MMP-2) by membrane type 1 matrix metalloproteinase through an artificial receptor for ProMMP-2 generates active MMP-2 [O] . Nishida, Y., Miyamori, H., Thompson, E.W., 2008

机译：膜型1基质金属蛋白酶通过ProMMP-2的人工受体激活基质金属蛋白酶2（MMP-2）产生活性MMP-2
8. Extracellular Matrix Regulations of Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) and Matrix Metalloproteinase-2 (MMP-2) in Human Breast Fibroblasts. [R] . Hernandez-Barrantes, S., Fridman, R. 2002

机译：人乳腺成纤维细胞膜型1基质金属蛋白酶（mT1-mmp）和基质金属蛋白酶-2（mmp-2）的细胞外基质调节。

Isolation and identification of canine matrix metalloproteinase-2 (MMP-2)

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