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首页> 外文期刊>The Veterinary Journal >Flow cytometric determination of oxidative burst activity of equineperipheral blood and bronchoalveolar lavage-derived leucocytes
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Flow cytometric determination of oxidative burst activity of equineperipheral blood and bronchoalveolar lavage-derived leucocytes

机译：流式细胞术测定等周血和支气管肺泡灌洗液衍生白细胞的氧化爆发活性

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摘要

Flow cytometric techniques were developed for the evaluation of oxidative burst activity in equine peripheral blood neutrophils and lymphocytes, as well as bronchoalveolar lavage derived pulmonary alveolar macrophages and lymphocytes. The oxidation of dichlorofluorescin was measured by the increased fluorescence of cells stimulated with phorbol myristate acetate or a variety of other stimulants. Flow cytometry was a suitable method for the evaluation of the intracellular oxidation in all cell populations evaluated. Analysis was rapid and cell separation before analysis was not required. Heterogenous cell populations with differing responsiveness to phorbol myristate acetate stimulated oxidative burst were identified in peripheral blood neutrophil and alveolar macrophage populations. The current study characterizes flow cytometric techniques for the evaluation of oxidative burst activity in equine peripheral blood and bronchoalveolar lavage-derived leucocytes.
机译：开发了流式细胞仪技术来评估马外周血中性粒细胞和淋巴细胞以及支气管肺泡灌洗液衍生的肺泡巨噬细胞和淋巴细胞中的氧化爆发活性。通过用佛波醇肉豆蔻酸酯乙酸盐或多种其他刺激物刺激的细胞的荧光增强来测量二氯荧光素的氧化。流式细胞术是评估所有被评估细胞群体中细胞内氧化的合适方法。分析迅速，不需要进行细胞分离。在外周血中性粒细胞和肺泡巨噬细胞群体中鉴定出对佛波醇肉豆蔻酸酯乙酸盐刺激的氧化爆发具有不同响应的异质细胞群体。当前的研究表征了流式细胞仪技术，用于评估马外周血和支气管肺泡灌洗液衍生的白细胞中的氧化爆发活性。

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