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首页> 外文期刊>Theriogenology >Expression of 3 beta-hydroxysteroid dehydrogenase, cytochrome P450 17alpha-hydroxylase/17,20-lyase and cytochrome P450 aromatase enzymes incorpora lutea of diestrous and early pregnant mares
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Expression of 3 beta-hydroxysteroid dehydrogenase, cytochrome P450 17alpha-hydroxylase/17,20-lyase and cytochrome P450 aromatase enzymes incorpora lutea of diestrous and early pregnant mares

机译：雌性和早孕母马黄体中3β-羟类固醇脱氢酶，细胞色素P45017α-羟化酶/ 17,20-裂合酶和细胞色素P450芳香化酶的表达

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In the pregnant mare, luteal estrogen production increases at the onset of equine chorionic gonadotropin (eCG) secretion by endometrial cups. In previous studies, we have demonstrated that eCG stimulates luteal androgen and estrogen production in pregnant mares. To further elucidate the regulation of steroidogenesis within the equine corpus luteum (CL) of pregnancy, we examined the expression of 3 beta -hydroxysteroid dehydrogenase (3 beta -HSD), cytochrome P450 17 alpha -hydroxylase/17,20 lyase (P450(17 alpha)) and cytochrome P450 aromatase (P450(arom)) in luteal tissue samples collected during diestrus (Days 7 to 10) and pregnancy before (Days 29 to 35) and after (Days 42 to 45) the onset of eCG secretion. Immunoblot analyses revealed a single protein per enzyme with molecular weights of 48 kDa (3 beta -HSD), 58 kDa (P450(17 alpha)) and 56 kDa (P450(arom)). Steady-state levels of 3 beta -HSD were lower in luteal tissue of diestrus than pregnancy, but expression did not change during pregnancy. Steady-state expression of P450(17 alpha) in CL of diestrus was not significantly different from that of pregnancy. During pregnancy, P450(17 alpha) expression was significantly higher after the onset of eCG secretion. Steady-state expression of P450(arom) in CL of diestrus was not significantly different from that of pregnancy. During pregnancy, luteal expression of P450(arom) was significantly lower after the onset of eCG secretion. These data support the hypotheses that eCG has a differential effect on the expression of luteal steroidogenic enzymes, that the eCG-induced increase in luteal estrogen production is the result of an increase in available aromatizable androgen due to an increase in P450(17 alpha) expression and activity, and that increased luteal estrogen production is not due to an increase in aromatase expression.
机译：在怀孕的母马中，子宫内膜杯分泌马绒毛膜促性腺激素（eCG）时，黄体雌激素产生增加。在以前的研究中，我们证明了eCG可以刺激怀孕母马的黄体雄激素和雌激素产生。为进一步阐明妊娠马黄体（CL）中类固醇生成的调控，我们检查了3β-羟类固醇脱氢酶（3 beta -HSD），细胞色素P450 17α-羟化酶/ 17,20裂解酶（P450（17 α））和细胞色素P450芳香化酶（P450（arom））在eCG分泌开始前（第29至35天）和妊娠（第29至35天）和之后（第42至45天）期间的黄体组织样品中收集的黄体组织样本中。免疫印迹分析揭示了每种酶的单一蛋白质，分子量分别为48 kDa（3 beta -HSD），58 kDa（P450（17 alpha））和56 kDa（P450（arom））。雌性黄体组织中3β-HSD的稳态水平低于妊娠，但在妊娠期间表达没有变化。 P450（17 alpha）在二重性CL中的稳态表达与妊娠期无明显差异。在怀孕期间，eCG分泌开始后，P450（17 alpha）表达明显升高。 P450（arom）在二重性CL中的稳态表达与妊娠无明显差异。怀孕期间，eCG分泌开始后，P450（arom）的黄体表达明显降低。这些数据支持以下假设：eCG对黄体类固醇生成酶的表达具有不同的影响，即eCG诱导的黄体雌激素产生的增加是由于P450（17 alpha）表达增加而可利用的可芳香化雄激素增加的结果和黄体活性增加，并不是因为芳香化酶表达的增加。

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