...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Thrombosis Research: An International Journal on Vascular Obstruction, Hemorrhage and Hemostasis >Effect of Armagnac fractions on human platelet aggregation in vitro and on rat arteriovenous shunt thrombosis in vivo probably not related only to polyphenols.
【24h】

Effect of Armagnac fractions on human platelet aggregation in vitro and on rat arteriovenous shunt thrombosis in vivo probably not related only to polyphenols.

机译：Armagnac馏分对体外人血小板聚集和体内对大鼠动静脉分流血栓形成的影响可能不仅与多酚有关。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previous studies showed that alcohol-free extracts of Armagnac, an oak cask aged spirit rich in polyphenols, inhibit human platelet function in vitro and in vivo, in an experimental rat arteriovenous shunt thrombosis model and in human healthy volunteers. To identify active compounds, we fractionated a freeze-dried extract of a 10-year-old Armagnac using successively chloroform, diethyl ether and ethyl acetate. The 4 resulting fractions were tested on in vitro human platelet aggregation induced by ADP and in vivo on arteriovenous shunt thrombosis after 10 days oral treatment in rats. Active components were found mainly in fractions 1 and 3: at the highest concentration (2.4 10(-2) g/l), in vitro ADP-induced aggregation was inhibited by 62.7+/-2.1% and 51.2+/-3.8% for F1 and F3, respectively, vs 18.9+/-2.4% and 13.9+/-0.4% for fractions 2 and 4 and 33.6+/-1.5% for the crude extract. There was a significant decrease in thrombus weight with the crude extract and all fractions tested after 10 days treatment with 2.5 mg/kg/day orally, greatest with fraction 1. Characterisation of phenol content showed that fraction 1, the most biologically active, was essentially devoid of ellagic acid and ellagitannins, the polyphenols initially thought responsible for the effect, whereas fraction 2 which was mostly inactive, was the richest in polyphenols. CONCLUSION: The antiplatelet and antithrombotic activity of Armagnac seems mostly unrelated to polyphenols.
机译：先前的研究表明，在实验性大鼠动静脉分流血栓形成模型和人体健康志愿者中，无酒提取物Armagnac（一种富含多酚的橡木桶陈年烈酒）可在体外和体内抑制人的血小板功能。为了鉴定活性化合物，我们使用氯仿，乙醚和乙酸乙酯分级分离了10年历史的Armagnac的冻干提取物。在大鼠口服治疗10天后，对4种所得级分进行了ADP诱导的体外人血小板凝集和体内动静脉分流血栓形成的测试。活性成分主要存在于级分1和3中：最高浓度（2.4 10（-2）g / l），体外ADP诱导的聚集被抑制了62.7 +/- 2.1％和51.2 +/- 3.8％。 F1和F3分别与馏分2和4的18.9 +/- 2.4％和13.9 +/- 0.4％以及粗提物的33.6 +/- 1.5％相比。粗提取物的血栓重量显着减少，口服2.5 mg / kg /天治疗10天后测试的所有馏分，馏分1表现最大。酚含量的表征表明，最具生物活性的馏分1基本上是由于缺乏鞣花酸和鞣花单宁，最初认为多酚是造成这种现象的原因，而大部分无活性的馏分2是多酚中含量最高的。结论：Armagnac的抗血小板和抗血栓形成活性似乎与多酚无关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号