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首页> 外文期刊>Tissue and Cell >The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering
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The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering

机译：由聚乳酸-乙醇酸/纤维蛋白杂种支架植入骨髓间充质干细胞构建的3维构建体在体外软骨组织工程中的潜力

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Articular cartilage is well known for its simple uniqueness of avascular and aneural structure that has limited capacity to heal itself when injured. The use of three dimensional construct in tissue engineering holds great potential in regenerating cartilage defects. This study evaluated the in vitro cartilaginous tissue formation using rabbit's bone marrow mesenchymal stem cells (BMSCs)-seeded onto poly(lactic-co-glycolic acid) PLGA/fibrin and PLGA scaffolds. The in vitro cartilaginous engineered constructs were evaluated by gross inspection, histology, cell proliferation, gene expression and sulphated glycosaminoglycan (sGAG) production at week 1, 2 and 3. After 3 weeks of culture, the PLGA/fibrin construct demonstrated gross features similar to the native tissue with smooth, firm and glistening appearance, superior histoarchitectural and better cartilaginous extracellular matrix compound in concert with the positive glycosaminoglycan accumulation on Alcian blue. Significantly higher cell proliferation in PLGA/fibrin construct was noted at day-7, day-14 and day-21 (p 0.05 respectively). Both constructs expressed the accumulation of collagen type II, collagen type IX, aggrecan and sox9, showed down-regulation of collagen type I as well as produced relative sGAG content with PLGA/fibrin construct exhibited better gene expression in all profiles and showed significantly higher relative sGAG content at each time point (p 0.05). This study suggested that with optimum in vitro manipulation, PLGA/fibrin when seeded with pluripotent non-committed BMSCs has the capability to differentiate into chondrogenic lineage and may serve as a prospective construct to be developed as functional tissue engineered cartilage. (C) 2015 Elsevier Ltd. All rights reserved.
机译：关节软骨以其简单的独特的无血管和神经结构而闻名，受伤时自愈能力有限。在组织工程中使用三维构建体在再生软骨缺损方面具有巨大潜力。这项研究评估了使用兔骨髓间充质干细胞（BMSCs）播种到聚乳酸-乙醇酸PLGA /纤维蛋白和PLGA支架上的体外软骨组织形成。在第1、2和3周通过肉眼检查，组织学，细胞增殖，基因表达和硫酸化糖胺聚糖（sGAG）产生来评估体外软骨工程构建体。培养3周后，PLGA /纤维蛋白构建体显示出与天然组织具有光滑，牢固和闪闪发光的外观，优越的组织结构和更好的软骨细胞外基质化合物，并与阿尔辛蓝上的正糖胺聚糖积聚在一起。在第7天，第14天和第21天，注意到在PLGA /纤维蛋白构建物中明显更高的细胞增殖（分别为p <0.05）。两种构建体均表达II型胶原蛋白，IX型胶原蛋白，聚集蛋白聚糖和sox9的积累，显示I型胶原蛋白的下调以及产生的相对sGAG含量，PLGA /纤维蛋白构建体在所有谱图中均表现出更好的基因表达，并且显示出相对较高的相对表达每个时间点的sGAG含量（p <0.05）。这项研究表明，通过最佳的体外操作，PLGA /纤维蛋白在接种多能性非承诺型BMSC时具有分化为软骨生成谱系的能力，并且可以用作功能性组织工程软骨的前瞻性构建体。 （C）2015 Elsevier Ltd.保留所有权利。

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