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首页> 外文期刊>Tissue engineering, Part A >In vitro differentiation and attachment of human embryonic stem cells on periodontal tooth root surfaces.
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In vitro differentiation and attachment of human embryonic stem cells on periodontal tooth root surfaces.

机译：人胚胎干细胞在牙周牙根表面的体外分化和附着。

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Periodontal tissue engineering based on cell replacement therapies is a promising field for improved regeneration of tooth supporting structures lost as a result of destructive periodontal diseases. Human embryonic stem cells (hESCs) could become adequate cell source for tissue engineering because of their unlimited proliferative potential and ability to differentiate to all somatic cell types. The aim of this study was to analyze the differentiation capacity of hESCs toward periodontal compartment cells and their relationship with tooth root surfaces in vitro. Periodontal ligament fibroblastic cell (PDLF) cultures were established and characterized; hESCs (HUES-9 line) were expanded in undifferentiated state and characterized for pluripotency morphologically and immunohistochemically. Extracted tooth root slices (RS) of 300 microm thickness, prepared with both periodontal and endodontic instrumentation, were used. Three different experimental groups were established: (i) undifferentiated hESC colonies cultured on and around the RS; (ii) undifferentiated hESC colonies cultured on and around RS with PDLF coculture, and (iii) undifferentiated hESC colonies cultured on and around RS with PDLF coculture in osteoinductive medium for 3 weeks. The fibrogenic and osteogenic marker expression was assessed with immunohistochemistry; histological staining and scanning electron microscopy were utilized to determine the relationship between differentiating hESCs and mineralized tooth root structures. Results demonstrate that hESC differentiation is influenced by tooth structures, PDLFs, and osteogenic medium, resulting with increased propensity toward mesenchymal lineage commitment, and formation of soft-hard tissue relationship in close contact areas. The proposed experimental system may facilitate further understanding in development of periodontal structures and contribute to realization of hESCs as a cell source in periodontal tissue engineering applications.
机译：基于细胞替代疗法的牙周组织工程是改善因破坏性牙周疾病而丧失的牙齿支撑结构再生的有前途的领域。人类胚胎干细胞（hESCs）可能具有无限的增殖潜力和分化为所有体细胞类型的能力，因此可以成为组织工程所需的充足细胞来源。这项研究的目的是分析体外胚胎干细胞对牙周细胞的分化能力及其与牙根表面的关系。建立并表征了牙周膜成纤维细胞（PDLF）培养物; hESCs（HUES-9系）在未分化状态下扩增，并在形态学和免疫组化方面鉴定了多能性。使用牙周和牙髓器械制备的300微米厚的提取牙根切片（RS）。建立了三个不同的实验组：（i）在RS及其周围培养的未分化hESC菌落; （ii）用PDLF共培养在RS上和周围培养的未分化hESC菌落，和（iii）用PDLF共培养在骨诱导培养基中在RS上和周围培养的未分化hESC菌落3周。用免疫组织化学评估纤维化和成骨标志物的表达。利用组织学染色和扫描电镜观察hESCs与矿化牙根结构之间的关系。结果表明，hESC的分化受牙齿结构，PDLF和成骨培养基的影响，导致对间充质沿袭承诺的倾向增加，并在紧密接触区域形成软硬组织关系。拟议的实验系统可能有助于进一步了解牙周结构的发展，并有助于实现hESCs作为牙周组织工程应用中的细胞来源。

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