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首页> 外文期刊>Tissue engineering, Part A >Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model
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Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model

机译：碎肉自体移植输注脱细胞骨骼肌的Codelivery改善了大鼠模型中体积性肌肉丢失的恢复。

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摘要

Skeletal muscle is capable of robust self-repair following mild trauma, yet in cases of traumatic volumetric muscle loss (VML), where more than 20% of a muscle's mass is lost, this capacity is overwhelmed. Current autogenic whole muscle transfer techniques are imperfect, which has motivated the exploration of implantable scaffolding strategies. In this study, the use of an allogeneic decellularized skeletal muscle (DSM) scaffold with and without the addition of minced muscle (MM) autograft tissue was explored as a repair strategy using a lower-limb VML injury model (n=8/sample group). We found that the repair of VML injuries using DSM+MM scaffolds significantly increased recovery of peak contractile force (81 +/- 3% of normal contralateral muscle) compared to unrepaired VML controls (62 +/- 4%). Similar significant improvements were measured for restoration of muscle mass (88 +/- 3%) in response to DSM+MM repair compared to unrepaired VML controls (79 +/- 3%). Histological findings revealed a marked decrease in collagen dense repair tissue formation both at and away from the implant site for DSM+MM repaired muscles. The addition of MM to DSM significantly increased MyoD expression, compared to isolated DSM treatment (21-fold increase) and unrepaired VML (37-fold) controls. These findings support the further exploration of both DSM and MM as promising strategies for the repair of VML injury.
机译：轻度创伤后，骨骼肌能够进行强大的自我修复，但是在创伤性体积性肌肉丢失（VML）的情况下，肌肉的质量损失超过20％，这种能力就会不堪重负。当前的自体全肌肉转移技术是不完善的，这激发了对植入式支架策略的探索。在这项研究中，探索了使用同种异体脱细胞骨骼肌（DSM）支架使用和不使用剁碎的肌肉（MM）自体移植组织的方法，作为使用下肢VML损伤模型的修复策略（n = 8 /样本组） ）。我们发现与未修复的VML对照（62 +/- 4％）相比，使用DSM + MM支架修复VML损伤显着提高了峰值收缩力（正常对侧肌肉的81 +/- 3％）的恢复。与未修复的VML对照（79 +/- 3％）相比，响应于DSM + MM修复的肌肉质量的恢复（88 +/- 3％）得到了类似的显着改善。组织学结果显示，对于DSM + MM修复的肌肉，在植入部位处和远离植入部位的胶原致密修复组织形成均明显减少。与单独的DSM处理（增加21倍）和未修复的VML（37倍）对照相比，在DSM中添加MM可以显着提高MyoD表达。这些发现支持对DSM和MM的进一步探索，作为修复VML损伤的有希望的策略。

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