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首页> 外文期刊>Toxicologic pathology >Gene expression in livers of BALB/C and C57BL/6J mice fed a high-fat diet
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Gene expression in livers of BALB/C and C57BL/6J mice fed a high-fat diet

机译：高脂饮食的BALB / C和C57BL / 6J小鼠肝脏中的基因表达

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We previously demonstrated that high-fat diet (HFD)-induced hepatic lipid accumulation is more severe in BALB/c mice than in C57BL/6J (B6) mice. To understand the changes in liver metabolism, we studied blood chemistry, gene expression, and histopathological changes of the liver in nine-week HFD-fed BALB/c and B6 mice and one- or four-week HFD-fed BALB/c mice. Serum total cholesterol and triglyceride levels were significantly increased in all HFD-fed groups, and one- and four-week HFD-fed BALB/c groups, respectively. Histopathology revealed that vacuolation of hepatocytes was severe in nine-week HFD-fed BALB/c mice, although it was less severe in the other groups. Microarray analysis of mRNA expression of nine-week HFD-fed BALB/c mice showed up-regulation of genes involved in fatty acid uptake and biosynthesis, such as Cd36, Acaca, Acly, and Fasn. Some changes were observed in the one- and four-week HFD-fed BALB/c groups and the nine-week HFD-fed B6 group, however these changes in mRNA expression were not so marked. In conclusion, the fatty accumulation observed in BALB/c mice may be caused, at least in part, by up-regulation of fatty acid uptake and biosynthesis. Cd36, Acaca, Acly and Fasn may be involved in these metabolic processes.
机译：我们先前证明，高脂饮食（HFD）诱导的肝脂质蓄积在BALB / c小鼠中比在C57BL / 6J（B6）小鼠中更为严重。为了了解肝脏代谢的变化，我们研究了在喂食9周的HFD的BALB / c和B6小鼠以及喂食1或4周的HFD的BALB / c小鼠中的血液化学，基因表达和肝脏的组织病理学变化。在所有由HFD喂养的组以及由HFD喂养的BALB / c组中，分别使血清总胆固醇和甘油三酯水平显着升高。组织病理学显示，在喂食HFD的9周BALB / c小鼠中，肝细胞的空泡化严重，而在其他组中则不那么严重。进行了为期9周的HFD喂养的BALB / c小鼠的mRNA表达的微阵列分析表明，参与脂肪酸摄取和生物合成的基因（例如Cd36，Acaca，Acly和Fasn）上调。在喂食HFD的BALB / c组为1周和4周，喂食FD6的B6组为9周，但观察到一些变化，但这些mRNA表达的变化并没有那么明显。总之，在BALB / c小鼠中观察到的脂肪蓄积可能至少部分地由脂肪酸摄取和生物合成的上调引起。 Cd36，Acaca，Acly和Fasn可能参与了这些代谢过程。

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