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首页> 外文期刊>Theoretical and Applied Genetics: International Journal of Breeding Research and Cell Genetics >Novel somatic hybrids (Solanum tuberosum L. plus Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight
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Novel somatic hybrids (Solanum tuberosum L. plus Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight

机译：新型体细胞杂种（Solanum tuberosum L. + Solanum tarnii）及其可育的BC1后代对马铃薯病毒Y和晚疫病表现出极强的抗性

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摘要

Solanum tarnii, a wild diploid, tuber-bearing Mexican species belonging to the series Pinnatisecta is highly resistant to Potato virus Y (PVY) and Colorado potato beetle and shows a strong hypersensitive reaction to Phytophthora infestans. Therefore, it could be a potential source of resistance to pathogens for potato breeders. S. tarnii (2n = 2x = 24) is reproductively isolated from tetraploid Solanum tuberosum and hence difficult to include in potato breeding programmes. In this study, interspecific somatic hybrids were produced for the first time by protoplast electrofusion of the cells of potato cv. Delikat (Solanum tuberosum L.) and Solanum tarnii. The hybrid nature of the regenerants was confirmed by simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers and by morphological analysis and flow cytometry. Selected somatic hybrids were successfully backcrossed with cv. Delikat. Parental lines, primary somatic hybrids and BC1 progeny were assessed for resistance to PVY by mechanical inoculation, grafting and exposure to viruliferous aphid vectors in the field, and resistance to late blight (P. infestans) by detached leaflet and whole tuber tests. The somatic hybrids showed no symptoms of viral infection and most of them displayed high levels of resistance to foliage blight. The BC1 progenies were highly resistant to PVY and a few were resistant to foliage blight. Selected hybrids and BC1 clones were evaluated in the field for tuber quality and tuber yield. Some BC1 clones produced yields of good quality tubers. The results confirm that both the resistance to PVY and to late blight of S. tarnii is expressed in somatic hybrids, and PVY resistance is transferred to BC1 progeny, whereas blight resistance is harder to transfer. Somatic hybridization again proved to be a valuable tool for producing pre-breeding material with increased genetic diversity.
机译：茄属茄属（Solanum tarnii）是属于Pinnatisecta系列的一种野生二倍体，带有块茎的墨西哥物种，对马铃薯病毒Y（PVY）和科罗拉多马铃薯甲虫具有高度抗性，并对疫霉疫霉表现出强烈的过敏反应。因此，它可能是马铃薯育种者对病原体抗性的潜在来源。塔恩链球菌（2n = 2x = 24）是从四倍体马铃薯的繁殖中分离出来的，因此很难纳入马铃薯育种计划。在这项研究中，种间体细胞杂种首次通过原生质体电融合马铃薯Cv细胞而产生。 Delikat（Solanum tuberosum L.）和Solanum tarnii。通过简单的序列重复（SSR）和扩增的片段长度多态性（AFLP）标记以及形态分析和流式细胞术证实了再生剂的杂种性质。选定的体细胞杂种成功地与简历回交。 Delikat。通过田间机械接种，嫁接和暴露于有毒蚜虫载体来评估亲本，初级体细胞杂种和BC1后代对PVY的抗性，并通过分离小叶和整个块茎试验评估对晚疫病（P. infestans）的抗性。体细胞杂种没有显示病毒感染的症状，并且大多数显示出对叶枯病的高水平抗性。 BC1后代对PVY具有高度抗性，少数对叶枯病具有抗性。在田间评估所选杂种和BC1克隆的块茎质量和块茎产量。一些BC1克隆产生了优质块茎。结果证实，塔氏链球菌对PVY和对晚疫病的抗性均在体细胞杂种中表达，PVY抗性转移至BC1后代，而抗白叶病则较难转移。体细胞杂交再次被证明是生产具有增加的遗传多样性的预育材料的有价值的工具。

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