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首页> 外文期刊>Tissue engineering, Part C. Methods >On the determination of Darcy permeability coefficients for a microporous tissue scaffold.
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On the determination of Darcy permeability coefficients for a microporous tissue scaffold.

机译：关于微孔组织支架的达西渗透系数的测定。

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Structural characterization of porous tissue scaffolds is challenging due to their complexity. Most investigators report the porosity of the material together with an estimate of the mean pore size and the pore size distribution. The usefulness of these measures is limited, especially in predicting the time-dependent permeation characteristics of a biodegradable, cell-seeded scaffold. A potential solution to this problem is to measure the permeability of the matrix and determine the Darcy permeability coefficient. Darcy permeability coefficients of 3.1 x 10(-14) and 6.3 x 10(-14) m(2) were measured for air and water, respectively, in microporous polycaprolactone scaffolds by monitoring fluid flow in response to a range of pressure differentials. Permeability coefficients for phosphate-buffered saline (5.3 x 10(-14) m(2)), glucose (5.7 x 10(-14) m(2)), and bovine serum albumin (1.8 x 10(-14) m(2)) were obtained by monitoring the change in concentration of molecular probes. This approach revealed the efficiency of transport of glucose molecules through the porous material and the existence of protein-scaffold interactions that resulted in protein retention and a reduction in fluid permeation rate. Darcy permeability measurements can provide valuable insights concerning the transport properties of nutrients, metabolites, and polypeptide growth factors in porous tissue engineering scaffolds and a method of quality assurance in scaffold processing.
机译：多孔组织支架的结构表征由于其复杂性而具有挑战性。大多数研究人员报告了材料的孔隙率，并估计了平均孔径和孔径分布。这些措施的实用性受到限制，尤其是在预测可生物降解的细胞播种支架的时间依赖性渗透特性方面。该问题的潜在解决方案是测量基质的渗透率并确定达西渗透率系数。通过监测流体流响应一定范围的压差，分别在微孔聚己内酯支架中测量到的空气和水的达西渗透系数分别为3.1 x 10（-14）和6.3 x 10（-14）m（2）。磷酸盐缓冲盐水（5.3 x 10（-14）m（2）），葡萄糖（5.7 x 10（-14）m（2））和牛血清白蛋白（1.8 x 10（-14）m（ 2））是通过监测分子探针浓度的变化而获得的。这种方法揭示了通过多孔材料转运葡萄糖分子的效率，以及存在蛋白质-支架相互作用的蛋白质-支架相互作用，这种相互作用导致蛋白质保留并降低了液体的渗透速率。达西渗透率测量可以提供有关多孔组织工程支架中营养物，代谢物和多肽生长因子的转运特性的有价值的见解，以及在支架加工中保证质量的方法。

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