• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tissue engineering, Part C. Methods >Preservation of porcine hepatocytes in three-dimensional bioreactor at room temperature using epigallocatechin-3-gallate
【24h】

Preservation of porcine hepatocytes in three-dimensional bioreactor at room temperature using epigallocatechin-3-gallate

机译：使用表没食子儿茶素-3-没食子酸酯在室温下在三维生物反应器中保存猪肝细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A bioartificial liver (BAL) assist system employing a three-dimensional (3D) bioreactor has been studied as a temporary support in liver failure. In the present study, a novel preservation method of primary cultured porcine hepatocytes in monolayer and 3D culture systems was studied. Epigallocatechin-3-gallate (EGCG), which has recently been found to have various bioactivities, was selected as a key compound for hepatocyte preservation. Hepatocytes isolated from porcine liver using the collagenase perfusion method were pre-cultured for 6 days, preserved at room temperature in the presence of EGCG at various concentrations for 4 days, and post-cultured in normal medium for another 6 days. In the monolayer culture, only albumin production rate was fully recovered after preservation when EGCG concentration was high (0.25 mg/mL). In contrast, albumin production and ammonium metabolism in the 3D bioreactor under the same condition recovered to 72±16% and 98±32%, respectively, of levels before preservation. These results indicate that hepatocytes can be preserved in the presence of 0.25 mg/mL of EGCG at room temperature, especially in a 3D culture system, which is promising technology for BAL preparation.
机译：已经研究了使用三维（3D）生物反应器的生物人工肝（BAL）辅助系统，作为肝衰竭的临时支持。在本研究中，研究了一种在单层和3D培养系统中保存原代培养猪肝细胞的新方法。最近发现具有多种生物活性的表没食子儿茶素-3-没食子酸酯（EGCG）被选作肝细胞保存的关键化合物。使用胶原酶灌注方法从猪肝分离的肝细胞预培养6天，在各种浓度的EGCG存在下于室温保存4天，然后在正常培养基中再培养6天。在单层培养中，当EGCG浓度高（0.25 mg / mL）时，保存后仅能完全恢复白蛋白产生速率。相反，在相同条件下3D生物反应器中白蛋白的产生和铵的代谢分别恢复到保存前的72±16％和98±32％。这些结果表明，在室温下，在0.25 mg / mL EGCG的存在下，肝细胞可以被保存，尤其是在3D培养系统中，这是制备BAL的有希望的技术。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号