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首页> 外文期刊>Tissue engineering, Part C. Methods >Regulating fibrinolysis to engineer skeletal muscle from the C2C12 cell line
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Regulating fibrinolysis to engineer skeletal muscle from the C2C12 cell line

机译：调节纤溶作用以工程化C2C12细胞系的骨骼肌

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Muscles engineered from transformed cells would be a powerful model for the study of muscle physiology by allowing long-term in vitro studies of muscle adaptation. However, previously described methods either take >5 weeks to produce a tissue or use collagen as a scaffold, which decreases the specific force of the muscle, making it hard to measure the function of the constructs. The aim of this study was to rapidly engineer muscle using the C2C12 cell line in fibrin, which has a stiffness similar to muscle tissue, allowing accurate functional testing. Both the protease inhibitor aprotinin and the natural cross-linker genipin increased the length of time that muscle could be cultured, with genipin increasing the time in culture to 10 weeks. The function of the tissues was significantly affected by the batch of serum (64-78%) or thrombin (41%), the differentiation medium (78%), and the seeding protocol (38%), but was unaffected by initial cell number. Strikingly, different C2C12 clones produced up to a 3.6-fold variation in force production. Under optimal conditions, the tissues form in 10.4±0.3 days and remain fully functional for 5 weeks over which time they continue to mature. The optimized model described here provides rapid, reliable, and functional tissues that will be useful in the study of skeletal muscle physiology.
机译：通过对肌肉适应性进行长期的体外研究，由转化细胞改造而成的肌肉将成为研究肌肉生理学的强大模型。然而，先前描述的方法或者花费> 5周来生产组织，或者使用胶原蛋白作为支架，这降低了肌肉的比力，使得难以测量构建体的功能。这项研究的目的是使用纤维蛋白中的C2C12细胞系快速工程改造肌肉，该细胞系具有类似于肌肉组织的硬度，可以进行准确的功能测试。蛋白酶抑制剂抑肽酶和天然交联剂Genipin均增加了肌肉的培养时间，而Genipin将培养时间延长至10周。批次的血清（64-78％）或凝血酶（41％），分化培养基（78％）和接种方案（38％）显着影响组织的功能，但不受初始细胞数的影响。令人惊讶的是，不同的C2C12克隆产生的力产生变化高达3.6倍。在最佳条件下，组织会在10.4±0.3天形成，并在5周内保持完全功能，在这段时间内它们会继续成熟。此处描述的优化模型提供了快速，可靠和功能强大的组织，可用于研究骨骼肌生理。

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