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首页> 外文期刊>Tissue engineering, Part C. Methods >The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation
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The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation

机译：基质血管部分和富血小板纤维蛋白在自体脂肪组织移植中的辅助应用

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Autologous adipose transplantation is rapidly gaining popularity for the restoration of soft tissue defects and lipoatrophy as well as for aesthetic improvements (e.g., facial reconstruction and rejuvenation). However, the current technique is crude that suffers from serious demerits, particularly the long-term unpredictability of volume maintenance due to resorption of the grafted adipose tissue and limited adipogenesis. We hypothesized that the adjuvant use of patient-derived adipose stromal vascular fraction (SVF) and platelet-rich fibrin (PRF) may enhance the overall outcome of autologous fat grafting in plastic and reconstructive surgery. Autologous SVF, with a mean cell number of (4.8??3.79)??107 cells/mL and a mean cell viability of 71.8%, and autologous PRF, with sustained release of multiple angiogenic growth factors, were created before surgical use. The following adipose tissue implants were injected subcutaneously into a rabbit ear's auricula according to the following study design: 2-mL adipose granules and 0.2-mL normal saline solution (AG+NS group), 2-mL adipose granules and 0.2-mL SVF (AG+SVF group), 2-mL adipose granules and 0.2mL PRF (AG+PRF group), or 2mL adipose granules combined with 0.1mL SVF and 0.1mL PRF (AG+SVF+PRF group). Histological examinations showed that the implanted adipose granules were well engrafted in the AG+SVF+PRF group, with a higher microvessel density 4 weeks postimplantation compared with the other three groups (p0.01). Twenty-four weeks postimplantation, the resorption rates of implanted tissue in each group were 49.39%??9.47%, 27.25%??4.37%, 36.41%??8.47%, and 17.37%??6.22%, respectively, and were significantly different (p0.01). The results demonstrated that the efficacy of adipose tissue implantation can be enhanced by using autologous PRF and SVF as therapeutic adjuvants, offering a clinically translatable strategy for soft tissue augmentation and reconstruction. ? 2012, Mary Ann Liebert, Inc. 2012.
机译：自体脂肪移植在软组织缺损和脂肪萎缩的修复以及美学改善（例如面部重建和嫩化）方面迅速普及。然而，目前的技术是粗制的，其具有严重的缺点，特别是由于移植的脂肪组织的再吸收和有限的脂肪形成，长期维持体积的不可预测性。我们假设，患者来源的脂肪间质血管分数（SVF）和富含血小板的纤维蛋白（PRF）的辅助使用可能会增强整形和重建手术中自体脂肪移植的总体效果。在手术前，先制备出平均细胞数为（4.8→3.79）×107细胞/ mL，平均细胞存活率为71.8％的自体SVF和具有多种血管生成生长因子持续释放的自体PRF。根据以下研究设计，将以下脂肪组织植入物皮下注射到兔耳的耳廓中：2 mL脂肪颗粒和0.2 mL生理盐水溶液（AG + NS组），2 mL脂肪颗粒和0.2 mL SVF（ AG + SVF组），2-mL脂肪颗粒和0.2mL PRF（AG + PRF组）或2mL脂肪颗粒与0.1mL SVF和0.1mL PRF组合（AG + SVF + PRF组）。组织学检查显示，AG + SVF + PRF组植入的脂肪颗粒植入良好，与其他三组相比，植入后4周的微血管密度更高（p <0.01）。植入后24周，各组的植入组织的吸收率分别为49.39％9.49.47％，27.25％?? 4.37％，36.41％?? 8.47％和17.37％?? 6.22％，并且显着差异（p <0.01）。结果表明，通过使用自体PRF和SVF作为治疗佐剂，可以增强脂肪组织植入的功效，为软组织的扩增和重建提供了可临床翻译的策略。 ？ 2012年，玛丽·安·利伯特（Mary Ann Liebert，Inc。）。2012年。

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