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MICROCALORIMETRIC INVESTIGATIONS ON THE INFLUENCE OF PROPOLIS ON THE BACTERIUM MICROCOCCUS LUTEUS

机译:蜂胶对细菌微球菌的影响的微量热研究

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Propolis or bee-glue is the third important product of honey bees after honey and wax. Known for thousands of years for its curative effects it is finding evermore attention in alternative and classical therapy in recent times against, e.g. infections, inflammations, dermatologic diseases and in wound healing. Microcalorimetric experiments were performed on the influence of several propolis samples from Germany, Uruguay, Ethiopia and of a commercial propolis tincture on growth and metabolism of the recommended gram-positive bacterium Micrococcus luteus. Propolis extracts were prepared following established recipies for the water-soluble and insoluble components and the precipitate. Addition of these extracts to a growing M. luteus culture in different growth phases resulted in a strong decline of the heat production rate, a prolongation of the lag phase or an introduction of a new, second lag phase, while the form of the calorimetric power-time curve remained unchanged. The calorimetric response showed a linear dependence on the propolis concentration. Although the quantitative gain of the extracts from the different propolis samples was nearly constant, the effects varied considerably between the specimens used. The calorimetric investigations were supplemented by polarographic oxygen monitoring and by the standard agar well technique to determine the growth inhibition factor of the propolis extracts. [References: 56]
机译:蜂胶或蜂胶是蜜蜂继蜂蜜和蜡之后的第三重要产品。数千年来,它以疗效着称,近年来在替代疗法和经典疗法中越来越受到关注,例如,感染,炎症,皮肤病和伤口愈合。对来自德国,乌拉圭,埃塞俄比亚的几种蜂胶样品和商业蜂胶tin剂对推荐的革兰氏阳性细菌黄体微球菌的生长和代谢的影响进行了量热实验。根据确定的水溶性和不溶性成分及沉淀物的配方制备蜂胶提取物。在不同生长阶段将这些提取物添加到正在生长的黄褐毛霉菌培养物中,导致发热量大幅下降,滞后阶段延长或引入了新的第二次滞后阶段,而量热形式时间曲线保持不变。量热响应显示对蜂胶浓度的线性依赖性。尽管从不同的蜂胶样品中提取的提取物的定量增益几乎是恒定的,但是在所使用的样品之间,效果却有很大差异。通过极谱氧监测和标准琼脂井技术补充量热研究,以确定蜂胶提取物的生长抑制因子。 [参考:56]

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