...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research >Formation of pyrophosphate-like adducts from nerve agents sarin, soman and cyclosarin in phosphate buffer: implications for analytical and toxicological investigations.
【24h】

Formation of pyrophosphate-like adducts from nerve agents sarin, soman and cyclosarin in phosphate buffer: implications for analytical and toxicological investigations.

机译：由神经毒药沙林，梭曼和环沙林在磷酸盐缓冲液中形成焦磷酸盐状加合物：对分析和毒理学研究的意义。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phosphate buffer is frequently used in biological, biochemical and biomedical applications especially when pH is to be controlled around the physiological value of 7.4. One of the prerequisites of a buffer compound among good buffering capacity and pH stability over time is its non-reactivity with other constituents of the solution. This is especially important for quantitative analytical or toxicological assays. Previous work has identified a number of amino alcohol buffers like TRIS to react with G-type nerve agents sarin, soman and cyclosarin to form stable phosphonic diesters. In case of phosphate buffer we were able to confirm not only the rapid hydrolysis of these agents to the respective alkyl methylphosphonates but also the formation of substantial amounts of pyrophosphate-like adducts (phosphorylated methylphosphonates), which very slowly hydrolyzed following zero-order kinetics. This led to a complex mixture of phosphorus containing species with changing concentrations over time. We identified the molecular structure of these buffer adducts using 1D (1)H-(3)(1)P HSQC NMR and LC-ESI-MS/MS techniques. Reaction rates of adduct formation are fast enough to compete with hydrolysis in aqueous solution and to yield substantial amounts of buffer adduct over the course of just a couple of minutes. Possible reaction mechanisms are discussed with respect to the formation and subsequent hydrolysis of the pyrophosphate-like compounds as well as the increased rate of hydrolysis of the nerve agent to the corresponding alkyl methylphosphonates. In summary, the use of phosphate buffer for the development of new assays with sarin, soman and cyclosarin is discouraged. Already existing protocols should be carefully reexamined on an individual basis.
机译：磷酸盐缓冲液经常用于生物，生物化学和生物医学领域，尤其是在将pH值控制在7.4的生理值附近时。良好的缓冲能力和随时间变化的pH稳定性中，缓冲化合物的先决条件之一是其与溶液中其他成分的不反应性。这对于定量分析或毒理学测定尤为重要。先前的工作已经确定了许多氨基醇缓冲液（如TRIS）可与G型神经毒剂沙林，梭曼和环沙林反应形成稳定的膦酸二酯。在磷酸盐缓冲液的情况下，我们不仅能够确认这些试剂迅速水解为各自的烷基甲基膦酸酯，而且还能确认形成大量的焦磷酸盐状加合物（磷酸化的甲基膦酸酯），其在零级动力学下非常缓慢地水解。这导致了含磷物质的复杂混合物，其浓度随时间变化。我们使用1D（1）H-（3）（1）P HSQC NMR和LC-ESI-MS / MS技术鉴定了这些缓冲液加合物的分子结构。加合物形成的反应速率足够快，足以与水溶液中的水解竞争，并在短短几分钟内产生大量的缓冲剂加合物。讨论了可能的反应机理，涉及焦磷酸盐类化合物的形成和随后的水解，以及神经毒剂水解为相应的烷基甲基膦酸酯的速率增加。总之，不建议将磷酸盐缓冲液用于沙林，梭曼和环沙林的新测定方法的开发。应该对现有协议进行仔细地重新检查。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号