Metabolomics analysis I. Selection of biological samples and practical aspects preceding sample preparation

B. Alvarez-Sanchez; F. Priego-Capote; M.D. Luque de Castro

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Metabolomics analysis I. Selection of biological samples and practical aspects preceding sample preparation

机译：代谢组学分析I.生物样品的选择和样品制备前的实际操作

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Metabolomics is one of the most recently emerged "-omics" sciences. Itssignificance in systems biology is gaining interest to levels similar toproteomics, transcriptomics and genomics. One of the main limitations inmetabolomics analysis is the lack of totally comprehensive approaches andin-depth studies, as individuals or laboratories with different skill sets usuallydevelop these. This variability particularly affects sample preparation due tothe extensive heterogeneity of biological samples. This two-part review aims to offer a practical guide to preparing biologicalsamples in metabolomics in order to reach the current level in genomics andproteomics. The first part focuses on analytical criteria for sample selectionand operations that precede sample preparation.

机译：代谢组学是最近出现的“-组学”科学之一。它在系统生物学中的重要性正在引起人们对与蛋白质组学，转录组学和基因组学相似的水平的关注。代谢组学分析的主要限制之一是缺乏完全综合的方法和深入的研究，因为具有不同技能的个人或实验室通常会开发这些方法。由于生物样品的广泛异质性，这种可变性特别影响样品制备。这份由两部分组成的综述旨在为制备代谢组学中的生物样品提供实用指南，以使基因组学和蛋白质组学达到当前水平。第一部分着重于样品制备之前的样品选择和操作的分析标准。

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《TrAC: Trends in Analytical Chemistry》 |2010年第2期|共9页
作者
B. Alvarez-Sanchez; F. Priego-Capote; M.D. Luque de Castro;
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正文语种 eng
中图分类分析化学;
关键词
Analytical criteria; Biological sample; Metabolite; Metabolomics; Samplepreparation; Sample selection; Sample storage;

机译：分析标准;生物样品;代谢物;代谢组学;样品制备;样品选择;样品存储;

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1. Metabolomics analysis I. Selection of biological samples and practical aspects preceding sample preparation [J] . B. Alvarez-Sanchez, F. Priego-Capote, M.D. Luque de Castro TrAC: Trends in Analytical Chemistry . 2010,第2期

机译：代谢组学分析I.生物样品的选择和样品制备前的实际操作
2. Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS I. Sample preparation (Review) [J] . Bischoff R., Bronsema K.J., van de Merbel N.C. TrAC: Trends in Analytical Chemistry . 2013,第Null期

机译：LC-MS / MS分析生物基质中的生物制药蛋白I.样品制备（综述）
3. Multi-dimensional, comprehensive sample extraction combined with LC-GC/MS analysis for complex biological samples: application in the metabolomics study of acute pancreatitis [J] . Yang Qin, Sun Jia, Chen Yong Q. RSC Advances . 2016,第31期

机译：多维，综合样品萃取与综合生物样品的LC-GC / MS分析相结合：在急性胰腺炎的代谢组科研究中的应用
4. Analytical Approaches for Sampling and Sample Preparation for Pesticides Analysis in Environmental, Food and Biological Samples [C] . Elisabeta Chirila, Camelia Draghici NATO advanced study institute on environmental security assessment and management of obsolete pesticides in Southeast Europe . 2013

机译：对环境，食品和生物样品杀虫剂分析的分析方法，用于杀虫剂分析
5. Applying Analytical Methods to Biological Sample Preparation and Analysis [D] . ?Sanders, Katherine Lee 2020

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6. Pursuing Experimental Reproducibility: An Efficient Protocol for the Preparation of Cerebrospinal Fluid Samples for NMR-Based Metabolomics and Analysis of Sample Degradation [O] . Benjamin Albrecht, Elena Voronina, Carola Schipke, 2020

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7. A new preparation method of calibration graphs for direct analysis of powdered biological samples by solid sampling atomic absorption spectrometry. Standard addition method for solid sampling technique. [O] . Ikuo ATSUYA, Hirotsugu MINAMI, Qiangbin ZHANG 1993

机译：一种新的制备方法，用于通过固体采样原子吸收光谱法直接分析粉末状生物样品的校准图。固体采样技术的标准添加方法。
8. I. Thermal Emission Resin Bead Mass Spectrometric Two-Filament Arrangement Evaluation - A.82. II. The Rapid Bulk Resin Bead Method of Sample Preparation for Mass Analysis of Plutonium and Uranium in Spent Reactor Fuel - A.56. [R] . McKown, H. S., Walker, R. L., Smith, D. H., 1981

机译：I.热发射树脂珠粒质谱双灯丝排列评估 - a.82。 II。用于反应堆燃料中钚和铀质量分析的快速散装树脂珠样品制备方法 - a.56。

Metabolomics analysis I. Selection of biological samples and practical aspects preceding sample preparation

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