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首页> 外文期刊>Transactions of the Royal Society of Tropical Medicine and Hygiene >Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP.
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Serodiagnosis of Leishmania donovani infections: assessment of enzyme-linked immunosorbent assays using recombinant L. donovani gene B protein (GBP) and a peptide sequence of L. donovani GBP.

机译：利什曼原虫多诺万尼感染的血清诊断：使用重组多诺尼诺氏菌基因B蛋白（GBP）和多诺氏菌GBP的肽序列评估酶联免疫吸附测定。

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摘要

The repetitive sequence of Leishmania major gene B protein (GBP) has previously been shown to be a useful tool in the diagnosis of cutaneous leishmaniasis (CL). Here, we have assessed enzyme-linked immunosorbent assays (ELISAs) using recombinant L. donovani GBP (rGBP) and a peptide sequence of L. donovani GBP (GBPP) in the diagnosis of L. donovani infections in Sudan. The sensitivity of the rGBP ELISA in diagnosing visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) was 92% and 93%, respectively. In contrast, the sensitivity of the GBPP ELISA was 55% for VL and 63% for PKDL. Plasma antibody reactivity of donors with VL and PKDL remained high for an extended period after the end of treatment. Antibody-reactivity to rGBP and GBPP was detected in 71% and 14% of plasma samples from CL patients, respectively. Plasma from healthy Sudanese donors living in an area endemic for malaria but free of leishmaniasis was negative in both assays.
机译：利什曼原虫主要基因B蛋白（GBP）的重复序列先前已被证明是诊断皮肤利什曼病（CL）的有用工具。在这里，我们评估了使用重组L. donovani GBP（rGBP）和L. donovani GBP（GBPP）的肽序列的酶联免疫吸附测定（ELISA），以诊断苏丹的L. donovani感染。 rGBP ELISA诊断内脏利什曼病（VL）和黑热病后皮肤利什曼病（PKDL）的敏感性分别为92％和93％。相反，GBPP ELISA对VL的敏感性为55％，对PKDL的敏感性为63％。治疗结束后，供体与VL和PKDL的血浆抗体反应性保持较高水平。在CL患者的血浆样本中分别检测到对rGBP和GBPP的抗体反应性为71％和14％。在这两种测定中，来自生活在疟疾流行地区但无利什曼病的健康苏丹供体的血浆均为阴性。

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