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首页> 外文期刊>Transactions of the Royal Society of Tropical Medicine and Hygiene >Skewing of cytokine profiles towards T helper cell type 2 response in visceral leishmaniasis patients unresponsive to sodium antimony gluconate.
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Skewing of cytokine profiles towards T helper cell type 2 response in visceral leishmaniasis patients unresponsive to sodium antimony gluconate.

机译：在对葡萄糖酸锑钠无反应的内脏利什曼病患者中，将细胞因子谱偏向T辅助细胞2型反应。

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Thirty-two parasitologically confirmed visceral leishmanisis (VL) patients and 23 healthy, age- and gender-matched controls were recruited between April 2001 and March 2002 and studied for intracellular cytokine production after stimulation in vitro using a Fluorescence Activated Cell Sorter-based intracellular cytokine assay. The VL patients were given i.m. sodium antimony gluconate at a dose of 20 mg/kg bodyweight daily for 28 d and were grouped as responders (n = 11) or non-responders (no response after 28 d of treatment; n = 21). Clinically, the non-responders had longer duration of illness (P < 0.05), larger spleen size (P < 0.05), and higher parasite load (P < 0.05) than responders. The percentage of T helper (Th) cells producing interferon-gamma (IFN-gamma) was significantly higher (P < 0.001) in responders than non-responders. Non-responders had higher IFN-gamma production than control subjects (P < 0.001). The percentage of Th cells producing interleukin-4 (IL-4) was significantly higher in non-responders than responders (P = 0.003) as well as in healthy subjects (P < 0.001). The frequency of IL-4 producing cells in responders and control subjects was similar (P= 0.65). The cytokine polarization index, as calculated by the formula loge IFN-gamma producing cells/loge IL-4 producing cells, was significantly lower in non-responders compared with both responders and control subjects (P = 0.003 and P < 0.001, respectively). The overall cytokine bias in non-responders was skewed towards a IL-4 dominance or Th2-like response and this was primarily due to induction of IL-4.
机译：在2001年4月至2002年3月之间，招募了32例被寄生虫学证实的内脏利什曼病（VL）患者和23名健康，年龄和性别匹配的对照，并使用基于荧光激活细胞分选仪的细胞内细胞因子体外刺激后研究了细胞内细胞因子的产生分析。 VL患者在当天早上接受治疗。每天剂量20 mg / kg体重的葡萄糖酸锑钠，持续28 d，分为反应者（n = 11）或无反应者（治疗28 d后无反应; n = 21）。临床上，无反应者的病程比反应者更长（P <0.05），脾脏大小更大（P <0.05），寄生虫负荷更高（P <0.05）。应答者中产生干扰素-γ（IFN-γ）的T辅助（Th）细胞百分比显着高于无应答者（P <0.001）。无反应者的IFN-γ产生高于对照组（P <0.001）。在无反应者中，产生白细胞介素4（IL-4）的Th细胞百分比显着高于对反应者（P = 0.003）和健康受试者（P <0.001）。应答者和对照者中产生IL-4的细胞的频率相似（P ＝ 0.65）。与反应者和对照组相比，由反应者logeIFN-γ产生细胞/ logIL-4产生细胞计算的细胞因子极化指数显着降低（分别为P = 0.003和P <0.001）。无反应者的总体细胞因子偏向于IL-4优势或类似Th2的反应，这主要归因于IL-4的诱导。

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