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首页> 外文期刊>Transactions of the Royal Society of Tropical Medicine and Hygiene >Comparison of three methods for the recovery of skin pathogens from impetigo swabs collected in a remote community of Northern Territory, Australia.
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Comparison of three methods for the recovery of skin pathogens from impetigo swabs collected in a remote community of Northern Territory, Australia.

机译：从澳大利亚北领地偏远社区收集的脓疱病拭子中回收皮肤病原体的三种方法的比较。

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Impetigo is a common infection in children living in remote areas. Immediate plating of impetigo swabs is the gold standard for bacterial recovery but is rarely feasible in remote regions. Bacterial culture increases our understanding of antibiotic resistance and strain diversity, which guides treatment protocols and epidemiological monitoring.We investigated three practical alternatives for recovering Streptococcus pyogenes and Staphylococcus aureus from transported swabs: dry swabs transported at 4°C with desiccant and plated within 48 h; swabs inoculated into skim milk tryptone glucose glycerol broth (STGGB), transported at 4°C, stored at -70°C and plated within 61 days; and ESwabs inoculated into Amies broth, transported at 4°C and plated within 48 h. Detection of Strep. pyogenes and Staph. aureus from simultaneously collected swabs was compared for the dry vs STGGB (36 sores) and the STGGB vs Amies (39 sores) methods. Swabs were collected from 43 children (75 sores sampled) in a remote community of Northern Territory, Australia in November 2011. The children had impetigo and were participating in the Skin Sore Trial [Australian Clinical Trials Registry ACTRN12609000858291].Recovery of Strep. pyogenes for dry vs STGGB was 72% (26/36) and 92% (33/36) and for STGGB vs Amies was 92% (36/39) for both methods. Staphylococcus aureus recovery for dry vs STGGB was 69% (25/36) and 72% 26/36) and for STGGB vs Amies was 74% (29/39) and 85% (33/39).STGGB and Amies media provided higher recovery of Strep. pyogenes than dry swabs. These results and the opportunity to batch and store specimens for molecular studies support the use of STGGB transport media for future impetigo research.
机译：脓疱疮是生活在偏远地区儿童的常见感染。立即接种脓疱疮拭子是细菌恢复的金标准，但在偏远地区很少可行。细菌培养提高了我们对抗生素耐药性和菌株多样性的了解，为治疗方案和流行病学监测提供了指导。我们研究了从运输的拭子中回收化脓性链球菌和金黄色葡萄球菌的三种可行替代方法：在4°C下运输干燥剂并在48 h内接种;将拭子接种到脱脂胰蛋白p葡萄糖甘油肉汤（STGGB）中，在4°C下运输，在-70°C下储存，并在61天内铺板;将ESwabs接种到Amies肉汤中，在4°C下运输并在48小时内铺板。检测链球菌。化脓和葡萄球菌。比较了同时采集的拭子的金黄色葡萄球菌的干法与STGGB法（36疮）和STGGB与Amies法（39疮）的比较。 2011年11月，在澳大利亚北领地的一个偏远社区中，从43名儿童（75疮的样本）中收集了拭子。这些儿童患有脓疱病，正在参加皮肤溃疡试验[澳大利亚临床试验注册中心ACTRN12609000858291]。链球菌的恢复。两种方法的干性与STGGB的化脓率分别为72％（26/36）和92％（33/36），而STGGB和Amies的化脓菌为92％（36/39）。相对于STGGB，干葡萄球菌的金黄色葡萄球菌回收率分别为69％（25/36）和72/26/36），对于STGGB和Amies，金黄色葡萄球菌的回收率分别为74％（29/39）和85％（33/39）。链球菌的恢复。化脓比干拭子。这些结果以及将标本进行批次和存储以进行分子研究的机会，为将STGGB转运介质用于未来脓疱病研究提供了支持。

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