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首页> 外文期刊>Transactions of the Royal Society of Tropical Medicine and Hygiene >Intestinal schistosomiasis in pre school-aged children of Lake Albert, Uganda: Diagnostic accuracy of a rapid test for detection of anti-schistosome antibodies
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Intestinal schistosomiasis in pre school-aged children of Lake Albert, Uganda: Diagnostic accuracy of a rapid test for detection of anti-schistosome antibodies

机译：乌干达艾伯特湖学龄前儿童的肠道血吸虫病：快速检测抗血吸虫病抗体的诊断准确性

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Background: A sensitive and reliable rapid diagnostic test (RDT) which should have comparable diagnostic performance against reference host serological methods is urgently needed for use in point-of-care (POC) diagnosis of intestinal schistosomiasis in pre school-aged children. Methods: The diagnostic accuracy of a RDT incorporating Schistosoma mansoni cercarial transformation fluid (SmCTF) for anti-schistosome antibody detection was evaluated with serum samples from a cohort of children fromUganda: 42 children aged under the age of 3 years and 40 children aged between 3 and 5 years. The infection status of these children had been previously determined by inspection of quadruplicate Kato-Katz faecal smears, a single urine circulating cathodic antigen (CCA) dipstick and antibody titres to S. mansoni soluble egg antigen (SmSEA) with a commercially available ELISA. Results: Upon comparison with quadruplicate Kato-Katz the sensitivity and specificity of the RDT were 75.7% and 31.1%, respectively. When using the SmSEA-ELISA as an alternate reference test, the RDT achieved 81.3% sensitivity and 61.1% specificity. Sensitivity and specificity compared to the urine-CCA test was 74.5% and 32.3% respectively. Sensitivity differed significantly according to age group. Conclusions: The performance of the RDT within this study appeared favourable when compared with the currently-available SmSEA-ELISA. Looking to the future a serological POC test would be particularly promising for use in disease mapping in younger children especially in guiding administration of praziquantel treatment in selective treatment settings.
机译：背景：用于学龄前儿童肠道血吸虫病的即时诊断（POC）诊断，迫切需要灵敏且可靠的快速诊断测试（RDT），其诊断性能应与参考宿主血清学方法相媲美。方法：使用来自乌干达的一组儿童的血清样本评估了掺入曼氏血吸虫曼氏血吸虫子宫颈转化液（SmCTF）的RDT对抗血吸虫抗体的诊断准确性：42名3岁以下儿童和40名3岁以下儿童和5年。这些孩子的感染状况先前已通过使用市售ELISA检查一式四份的Kato-Katz粪便涂片，单个尿循环阴极抗原（CCA）量油尺和曼氏梭菌可溶性蛋抗原（SmSEA）抗体滴度来确定。结果：与一式四份的Kato-Katz相比，RDT的敏感性和特异性分别为75.7％和31.1％。当使用SmSEA-ELISA作为替代参考测试时，RDT达到了81.3％的灵敏度和61.1％的特异性。与尿液-CCA检测相比，敏感性和特异性分别为74.5％和32.3％。敏感性因年龄组而有显着差异。结论：与当前可用的SmSEA-ELISA相比，该研究中RDT的性能似乎良好。展望未来，血清学POC检测将特别有望用于年幼儿童的疾病作图，特别是在选择性治疗环境中指导吡喹酮治疗的指导。

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