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首页> 外文期刊>Transactions of the Royal Society of Tropical Medicine and Hygiene >Detection of Cryptosporidium and Giardia (oo)cysts by IFA, PCR and LAMP in surface water from Rasht, Iran.
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Detection of Cryptosporidium and Giardia (oo)cysts by IFA, PCR and LAMP in surface water from Rasht, Iran.

机译：通过IFA，PCR和LAMP在伊朗拉什特的地表水中检测隐孢子虫和贾第鞭毛虫（oo）囊肿。

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Cryptosporidium and Giardia in water supplies is acknowledged as a public health problem. In the present study, we applied immunofluorescence assay (IFA), PCR and loop-mediated isothermal amplification (LAMP) for the detection of the two protozoa.Over a period of 12 months, surface water samples were collected from two rivers in the north of Iran, and filtrated by 142 mm membrane filters. At each sampling point 10 L water were used for IFT and the10 L were analysed using molecular methods.In 15/40 samples, (oo)cysts were detected by one of the IFA, PCR or LAMP methods. Five samples that were Cryptosporidium-negative by IFA were positive by LAMP. A total of 10 out of 13 samples that were Giardia-positive by IFA were also positive by PCR. IFA revealed high levels of Giardia, with 1-1800 cysts and 1-16 Cryptosporidium oocysts detected per 10 L.The study reveals that the investigated water supplies were contaminated by Cryptosporidium and Giardia. The LAMP assay has advantages for detection and screening of these protozoa at relatively low concentration in water samples. The three assays applied are complimentary but no single one will give the true prevalence of these parasites in surface water samples. However, each method has its own advantages and disadvantages dependent of the aim and the study design; a combination of detection methods should be applied to discover whether water is, or is not, contaminated with (oo)cysts. This is the first report on the occurrence of (oo)cysts in Iranian surface waters to compare the results of parasite detection obtained with the different methods.
机译：供水中的隐孢子虫和贾第鞭毛虫是公认的公共卫生问题。在本研究中，我们应用免疫荧光测定（IFA），PCR和环介导的等温扩增（LAMP）来检测这两个原生动物。在12个月的时间里，从北部的两条河流收集了地表水样品伊朗，并用142毫米膜滤器过滤。在每个采样点，将10 L水用于IFT，并使用分子方法进行分析。在15/40个样品中，通过IFA，PCR或LAMP方法之一检测到（oo）囊肿。 LAMP对IFA隐孢子虫阴性的5个样品呈阳性。通过IFA，贾第鞭毛虫阳性的13个样品中，共有10个也呈阳性。 IFA显示出高水平的贾第鞭毛虫，每10 L检测到1-1800个囊肿和1-16个隐孢子虫卵囊。研究表明，所调查的供水受到隐孢子虫和贾第鞭毛虫的污染。 LAMP测定法在检测和筛选水样品中浓度相对较低的原生动物方面具有优势。所应用的三种测定是互补的，但没有一个能真正反映出地表水样品中这些寄生虫的普遍性。但是，每种方法都有各自的优缺点，这取决于目的和研究设计。应当结合使用多种检测方法来发现水是否被（卵）囊污染。这是有关伊朗地表水中（oo）囊肿发生的第一份报告，比较了用不同方法获得的寄生虫检测结果。

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