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首页> 外文期刊>Toxicology: An International Journal Concerned with the Effects of Chemicals on Living Systems >Protective effect of sulforaphane pretreatment against cisplatin-induced liver and mitochondrial oxidant damage in rats.
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Protective effect of sulforaphane pretreatment against cisplatin-induced liver and mitochondrial oxidant damage in rats.

机译：萝卜硫烷预处理对顺铂诱导的大鼠肝脏和线粒体氧化损伤的保护作用。

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摘要

In the present work was analyzed whether sulforaphane (SFN) may protect against cisplatin (CIS)-induced hepatic damage, oxidant stress and mitochondrial dysfunction. Four groups of male Wistar rats were studied: control, CIS, CIS+SFN and SFN. SFN was given i.p. (500 mug/kg/d x 3 days) before CIS administration (single i.p. injection, 10mg/kg). Rats were sacrificed 3 days after CIS injection to evaluate hepatic damage (histological analysis, liver/body weight ratio and serum activity of aspartate aminotransferase and alanine aminotransferase), oxidant stress (lipid peroxidation and protein carbonyl and glutathione content), antioxidant enzymes (catalase, glutathione reductase, glutathione peroxidase, glutathione-S-transferase and superoxide dismutase) in liver homogenates and isolated mitochondria and mitochondrial function (oxygen consumption using either malate/glutamate or succinate as substrates and the activity of mitochondrial complex I, II, II-III, IV and V). Furthermore it was evaluated if SFN is able to scavenge some reactive oxygen species in vitro. It was found that SFN prevents CIS-induced (a) hepatic damage, (b) oxidant stress and decreased activity of antioxidant enzymes in liver and mitochondria and (c) mitochondrial alterations in oxygen consumption and decreased activity of mitochondrial complex I. It was also found that the scavenging ability of SFN for peroxynitrite anion, superoxide anion, singlet oxygen, peroxyl radicals, hydrogen peroxide and hydroxyl radicals was very low or negligible. The hepatoprotective effect of SFN was associated to the preservation of mitochondrial function, antioxidant enzymes and prevention of liver and mitochondrial oxidant stress.
机译：在目前的工作中分析了萝卜硫烷（SFN）是否可以防止顺铂（CIS）诱导的肝损伤，氧化应激和线粒体功能障碍。研究了四组雄性Wistar大鼠：对照，CIS，CIS + SFN和SFN。 SFN的腹膜内注射（500杯/公斤/天x 3天），然后再服用CIS（一次腹膜内注射，每次10毫克/公斤）。 CIS注射3天后处死大鼠以评估肝损伤（组织学分析，肝/体重比和天冬氨酸转氨酶和丙氨酸转氨酶的血清活性），氧化应激（脂质过氧化和蛋白质羰基和谷胱甘肽含量），抗氧化酶（过氧化氢酶，肝匀浆中的谷胱甘肽还原酶，谷胱甘肽过氧化物酶，谷胱甘肽-S-转移酶和超氧化物歧化酶）和分离的线粒体和线粒体功能（以苹果酸/谷氨酸或琥珀酸酯为底物的氧气消耗以及线粒体复合物I，II，II-III的活性） IV和V）。此外，评估了SFN是否能够在体外清除某些活性氧。已发现SFN可以预防CIS引起的（a）肝损伤，（b）氧化应激和肝和线粒体中抗氧化酶活性的降低以及（c）线粒体耗氧量的改变和线粒体复合体I的活性降低。发现SFN对过氧亚硝酸根阴离子，超氧阴离子，单线态氧，过氧自由基，过氧化氢和羟​​基自由基的清除能力非常低或可忽略不计。 SFN的保肝作用与线粒体功能的维持，抗氧化酶以及肝和线粒体氧化应激的预防有关。

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