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首页> 外文期刊>Toxicon: An International Journal Devoted to the Exchange of Knowledge on the Poisons Derived from Animals, Plants and Microorganisms >A comparative study of biological activities of crotoxin and CB fraction of venoms from Crotalus durissus terrificus, Crotalus durissus cascavella and Crotalus durissus collilineatus
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A comparative study of biological activities of crotoxin and CB fraction of venoms from Crotalus durissus terrificus, Crotalus durissus cascavella and Crotalus durissus collilineatus

机译：猪屎豆，猪屎豆和猪屎豆的毒液中cro毒素和CB部分生物活性的比较研究

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In Brazil, the Crotalus durissus terrificus subspecie is the most studied, particularly concerning its crotoxin. Crotoxin is the major toxic component of the South American rattlesnake Crotalus durissus venom. It is composed of two different subunits, CA called crotapotin and CB weakly toxic phospholipase A(2) with high enzymatic activity. In this paper, we decided to make a study of the main toxic characteristics of crotoxin (CTX) and CB fraction from the other subspecies, Crotalus durissus cascavella and of Crotalus durissus collilineatus, in comparison with those of C. d. terrificus. Ours results have shown that the venoms presented similar chromatographic profiles and the purified fractions were free of contaminants. Regarding the toxic activities, the DL50 of the crotoxins showed no significant differences between the subspecies. The smaller toxicity of CB indicated that the toxicity of the crotoxin complex depends on the interaction between CA and CB. CTX and fraction CB of the three species of Crotalus showed negligible proteolytic activity. C. d. terrificus CTX presented higher PLA(2) activity when compared with the others two subspecies. The oedema induced by CB developed later than the CTX and reached its peak 3 It after the injection. The myotoxic activity was determined by assaying serum CK levels. Mice injected with CTX of C. d. terrificus presented greater myotoxic activity compared to the others. The myotoxic activity of CB from the three subspecies was lower than the activity of the crotoxin, reinforcing the idea that the fraction CA increases the toxicity of CB.
机译：在巴西，对Crotarus durissus terrificus亚种的研究最为深入，特别是关于其croto毒素。 Crotoxin是南美响尾蛇Crotalus durissus毒液的主要有毒成分。它由两个不同的亚基，称为crotapotin的CA和具有高酶活性的CB弱毒性磷脂酶A（2）组成。在本文中，我们决定对其他亚种Crotusus durissus cascavella和Crotalus durissus collilineatus的crotoxin（CTX）和CB馏分的主要毒性特征进行比较研究。很棒我们的结果表明，毒液的色谱图相似，​​纯化后的馏分不含污染物。关于毒性活性，crotoxins的DL50在亚种之间没有显着差异。 CB的毒性较小，表明crotoxin复合物的毒性取决于CA和CB之间的相互作用。三种猪屎豆的CTX和CB组分的蛋白水解活性可忽略不计。 d。与其他两个亚种相比，terrificus CTX表现出更高的PLA（2）活性。由CB引起的水肿比CTX迟发，并在注射后达到其峰值3 It。通过测定血清CK水平确定肌毒性活性。注射CTX的小鼠d。与其他相比，terrificus表现出更大的肌毒性活性。来自这三个亚种的CB的肌毒性活性低于crotoxin的活性，从而强化了CA组分增加CB毒性的观点。

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