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首页> 外文期刊>Toxicon: An International Journal Devoted to the Exchange of Knowledge on the Poisons Derived from Animals, Plants and Microorganisms >Development of a sensitive enzyme-linked immunosorbent assay for the detection of fumonisin B-1 in maize
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Development of a sensitive enzyme-linked immunosorbent assay for the detection of fumonisin B-1 in maize

机译：用于检测玉米中伏马毒素B-1的酶联免疫吸附测定的灵敏性

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摘要

Fumonisin B1 (FB1) is a mycotoxin, mainly produced by Fusarium fungi and present in food and feed. It causes harmful effects on human and animal health. Therefore, it is necessary to develop sensitive and reliable screening methods. In this study, a highly sensitive monoclonal antibody (MAb) against FB1, clone 2D7, was produced, and the 50% inhibition concentration (IC50) of the MAb was 2.2 ng/mL in buffer. The MAb showed high cross-reactivity with fumonisin B2 (FB2), and negligible cross-reactivity with other mycotoxins. A sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) based on this MAb was developed for the determination of FB1 in maize. In spiked samples (100, 200 and 500 micro g/kg), the average recoveries ranged from 78+or-11 to 107+or-4%, and the coefficient of variation ranged from 3 to 15%. The limit of detection of the icELISA was 5.4 micro g/kg. This method was compared to liquid chromatography tandem mass spectrometry (LC-MS/MS) using naturally contaminated samples, and the correlation coefficient was above 0.82. These results show the reliability of the icELISA method for the determination of FB1 in maize.
机译：伏马菌素B 1 （FB 1 ）是一种真菌毒素，主要由镰刀菌真菌产生，存在于食品和饲料中。它对人类和动物健康造成有害影响。因此，有必要开发灵敏可靠的筛选方法。在这项研究中，产生了针对FB 1 的高度敏感的单克隆抗体（MAb）2D7，其单克隆抗体的50％抑制浓度（IC 50 ）为缓冲液中2.2 ng / mL。 MAb与伏马毒素B 2 （FB 2 ）的交叉反应性很高，与其他霉菌毒素的交叉反应性可以忽略不计。建立了基于这种单克隆抗体的敏感的间接竞争酶联免疫吸附测定法（icELISA），用于测定玉米中的FB 1 。在加标样品（100、200和500 micro g / kg）中，平均回收率在78％或-11％至107 + or-4％之间，变异系数在3％至15％之间。 icELISA的检出限为5.4 micro g / kg。与使用自然污染样品的液相色谱串联质谱法（LC-MS / MS）进行了比较，相关系数在0.82以上。这些结果证明了icELISA法测定玉米中FB 1 的可靠性。

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