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首页> 外文期刊>Transactions of the American Ophthalmological Society. >Experimental nonenzymatic glycosylation of vitreous collagens occurs by two pathways.
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Experimental nonenzymatic glycosylation of vitreous collagens occurs by two pathways.

机译：玻璃体胶原蛋白的实验性非酶糖基化通过两种途径发生。

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摘要

PURPOSE: To study the process of nonenzymatic glycosolation of vitreous collagen in vitro to determine the contributions of the classic Maillard pathway and the oxidative pathway, as well as to evaluate possible inhibitors of both pathways. METHODS: Bovine vitreous collagen was extracted and then incubated with hexoses in vitro. The amount of advanced glycosylation end (AGE) products was measured by fluorometry under varying conditions in the presence and absence of glycosolation inhibitors. Oxygen consumption studies and electron spin resonance spectroscopy with and without free-radical inhibitors were performed to differentiate oxidative from nonoxidative glycosolation. RESULTS: Vitreous collagen undergoes nonenzymatic glycosolation in the presence of glucose or galactose in vitro. Oxygen consumption data show that oxygen is consumed in glucose and galactose solutions. Oxygen consumption is decreased by known free-radical inhibitors and rutin but not aminoguanidine. Electron spin resonance spectroscopy demonstrated the presence of a carbon-centered radical, and known free-radical inhibitors decreased the carbon-centered signal. CONCLUSIONS: Nonenzymatic glycosolation of vitreous collagen can occur not only by the classic nonoxidative pathway, but also by a second oxidative pathway that is susceptible to a number of inhibitors.
机译：目的：研究玻璃体胶原蛋白的非酶促糖基化过程，以确定经典美拉德途径和氧化途径的作用，并评估两种途径的可能抑制剂。方法：提取牛玻璃体胶原蛋白，然后与己糖体外孵育。在存在和不存在糖基化抑制剂的条件下，通过荧光法在不同条件下测量高级糖基化末端（AGE）产物的量。进行了有氧抑制剂和无氧抑制剂的耗氧量研究和电子自旋共振光谱分析，以区分氧化性糖化和非氧化性糖化。结果：玻璃体胶原在葡萄糖或半乳糖存在的条件下经历了非酶糖基化。耗氧量数据显示，葡萄糖和半乳糖溶液消耗了氧气。已知的自由基抑制剂和芦丁可减少氧气消耗，而氨基胍则不会。电子自旋共振光谱证明存在以碳为中心的自由基，并且已知的自由基抑制剂降低了以碳为中心的信号。结论：玻璃态胶原的非酶糖基化不仅可以通过经典的非氧化途径发生，而且可以通过对许多抑制剂敏感的第二种氧化途径发生。

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