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首页> 外文期刊>Transactions of the American Ophthalmological Society. >Collagen type I and III synthesis by Tenon's capsule fibroblasts in culture: individual patient characteristics and response to mitomycin C, 5-fluorouracil, and ascorbic acid.
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Collagen type I and III synthesis by Tenon's capsule fibroblasts in culture: individual patient characteristics and response to mitomycin C, 5-fluorouracil, and ascorbic acid.

机译：Tenon胶囊成纤维细胞在培养中合成的I型和III型胶原：患者的个体特征以及对丝裂霉素C，5-氟尿嘧啶和抗坏血酸的反应。

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PURPOSE: This study was performed to better understand the differences between patients in specific components of wound healing as it may pertain to glaucoma filtration surgery, including the use of antimetabolites. METHODS: Human Tenon's capsule fibroblasts were obtained at the time of glaucoma filtering surgery and established in individual cell cultures from 35 glaucoma patients. The dose-response to 5-fluorouracil (5FU) and mitomycin C (MMC) was determined. The individual cell lines were exposed to the antimetabolites and ascorbic acid with measurement of collagen type I and III production by an ELISA-type dot blot assay. These results were then statistically compared to the individual patient characteristics including age, race, previous surgery and medications, and type of glaucoma. RESULTS: 5-FU had little effect on collagen type I and III production or protein synthesis. MMC had an inhibitory effect on collagen secretion and total protein synthesis with increasing concentration. Photomicrographs of the cells after each treatment condition revealed characteristic morphologic changes when compared to controls. There was a large range of collagen type I and III production with correlation between the amounts of each collagen type secreted in response to the antimetabolites. However, there was no correlation with accepted risk factors for filtration failure. CONCLUSION: These antimetabolites act similarly on different cell lines in a nonspecific manner. The results suggest that the increased risk of filtration failure due to age, race, diagnosis, and previous conjunctival surgery is not due to differences in secretion of collagen types I and III by Tenon's capsule fibroblasts.
机译：目的：进行这项研究是为了更好地了解患者在伤口愈合的特定组成部分之间的差异，因为这可能与青光眼滤过手术有关，包括使用抗代谢物。方法：从青光眼滤过手术时获得人腱囊成纤维细胞，并在35名青光眼患者的单个细胞培养物中建立。确定了对5-氟尿嘧啶（5FU）和丝裂霉素C（MMC）的剂量反应。将各个细胞系暴露于抗代谢物和抗坏血酸，并通过ELISA型斑点印迹法测量I型和III型胶原的产生。然后将这些结果与各个患者的特征进行统计学比较，包括年龄，种族，先前的手术和药物治疗以及青光眼的类型。结果：5-FU对I型和III型胶原蛋白的产生或蛋白质合成影响很小。随着浓度的增加，MMC对胶原蛋白的分泌和总蛋白的合成具有抑制作用。与对照相比，每种处理条件后细胞的显微照片显示出特征性的形态变化。 I型和III型胶原蛋白的生产范围很大，每种对抗代谢物的胶原蛋白分泌的量之间存在相关性。但是，与公认的过滤失败风险因素没有相关性。结论：这些抗代谢物以不同的方式作用于不同的细胞系。结果表明，由于年龄，种族，诊断和先前的结膜手术而导致滤过失败的风险增加不是由于Tenon胶囊成纤维细胞分泌的I型和III型胶原蛋白的差异。

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