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首页> 外文期刊>Transactions of the American Ophthalmological Society. >Keratocyte density in vivo after photorefractive keratectomy in humans.
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Keratocyte density in vivo after photorefractive keratectomy in humans.

机译：人类光折射性角膜切除术后体内的角质形成细胞密度。

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摘要

PURPOSE: To determine changes in keratocyte density in central human corneas in vivo after photorefractive keratectomy (PRK). METHODS: Fifteen patients (25 eyes) received excimer PRK (VISX Star) with epithelial removal by laser-scrape (43 microns ablation followed by manual scrape) to correct myopia between -1.5 D and -7.25 D. Corneas were examined by using confocal microscopy in vivo before PRK and at 1 day, 5 days, 1 month, and 3 months after PRK. A custom automated image-processing algorithm identified bright objects (keratocytes) against a dark background and estimated keratocyte density by using the number and size of the objects. Cell density was quantified in anteroposterior stromal regions after PRK and compared to cell density in corresponding pre-PRK regions. RESULTS: One day after PRK, keratocyte density increased 9% in the anterior third of the stroma (pi = .003), was unchanged in the middle third of the stroma (pi = .481), and decreased 6% in the posterior third of the stroma (pi = .038). Keratocyte density remained elevated in the anterior stroma to 3 months after PRK; at this time, there was a 13% increase in keratocyte density throughout the full-thickness stroma (pi < .001). CONCLUSIONS: Keratocyte density was increased in the anterior stroma immediately after PRK in humans. Three months later, keratocyte density was increased in all anteroposterior stromal regions, suggesting that PRK affects keratocytes throughout the entire central cornea.
机译：目的：确定屈光性角膜切除术（PRK）后体内人中央角膜的角膜细胞密度变化。方法：15例患者（25眼）接受准分子PRK（VISX Star），并通过激光刮除（43微米消融，然后手动刮除）上皮去除，以矫正近视度在-1.5 D和-7.25 D之间。通过共聚焦显微镜检查角膜在PRK之前和PRK术后1天，5天，1个月和3个月的体内。一种定制的自动图像处理算法可以在黑暗的背景下识别明亮的物体（角质形成细胞），并通过使用物体的数量和大小来估计角质形成密度。在PRK后，在前后基质区域对细胞密度进行定量，并与相应的PRK前区域的细胞密度进行比较。结果：PRK一天后，间质前三分之一（pi = .003）的角膜细胞密度增加了9％，而中间三分之一（pi = .481）的角质细胞密度没有变化，而后三分之一的角膜细胞密度降低了6％的间质（pi = .038）。到PRK后3个月，前间质中的角质细胞密度仍保持升高。此时，整个全层基质的角膜细胞密度增加了13％（pi <.001）。结论：人PRK后即刻前基质中的角质形成细胞密度增加。三个月后，所有前后基质区域的角膜细胞密度均增加，表明PRK影响整个角膜中央的角膜细胞。

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