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首页> 外文期刊>Translational research: the journal of laboratory and clinical medicine >Plexin B1 is downregulated in renal cell carcinomas and modulates cell growth.
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Plexin B1 is downregulated in renal cell carcinomas and modulates cell growth.

机译：Plexin B1在肾细胞癌中下调并调节细胞生长。

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Plexins are a family of transmembrane receptors that interact with the repulsive axon guidance molecules (Semaphorins) in neural tissues. In extraneural tissues, plexins are involved in other cellular functions often altered in neoplastic cells, such as adhesion, migration, and apoptosis. Plexin B1 has been implicated in the regulation of Akt, which is an activated pathway in renal cell neoplasms, and only 1 report has emphasized its role as an oncogenic factor. Furthermore, plexin B1 is located in 3p21, which is a chromosomal region deleted frequently in renal cell carcinomas. In accordance with a hypothetical oncogenic role for plexin B1, we have shown by reverse transcription-polymerase chain reaction that plexin B1 is expressed in nonneoplastic renal tissue, and it is severely downregulated in clear cell renal carcinomas. We have also demonstrated by immunohistochemistry on tissue microarrays that plexin B1 protein is absent in more than 80% of renal cell carcinomas (169 in 209 carcinomas examined). Otherwise, all kinds of renal tubules showed strong membrane reactivity. Moreover, when we have induced plexin B1 expression with an expression vector in the renal adenocarcinoma cell line ACHN, a marked reduction in proliferation rate was produced. Altogether, this evidence suggests a possible role for plexin B1 in renal oncogenesis.
机译：丛蛋白是跨膜受体的家族，其与神经组织中的排斥轴突引导分子（Semaphorins）相互作用。在神经外组织中，丛蛋白还参与其他通常在赘生性细胞中发生改变的细胞功能，例如粘附，迁移和凋亡。 Plexin B1与Akt的调节有关，Akt是肾细胞肿瘤中的一条活化途径，只有1篇报告强调了其作为致癌因子的作用。此外，丛蛋白B1位于3p21，这是在肾细胞癌中经常缺失的染色体区域。根据假定的plexin B1致癌作用，我们通过逆转录聚合酶链反应显示plexin B1在非肿瘤性肾组织中表达，并且在透明细胞肾癌中严重下调。我们还通过免疫组织化学在组织微阵列上证实，超过80％的肾细胞癌（检查的209种癌中有169种）不存在plexin B1蛋白。否则，各种肾小管均表现出较强的膜反应性。而且，当我们用表达载体诱导plexin B1表达在肾腺癌细胞株ACHN中时，增殖速率明显降低。总之，这一证据表明plexin B1在肾癌发生中可能发挥作用。

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