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首页> 外文期刊>Translational research: the journal of laboratory and clinical medicine >In vitro cultivation of islet-like cell clusters from human umbilical cord blood-derived mesenchymal stem cells.
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In vitro cultivation of islet-like cell clusters from human umbilical cord blood-derived mesenchymal stem cells.

机译：从人脐带血来源的间充质干细胞体外培养胰岛样细胞簇。

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摘要

A major obstacle to successful islet transplantation for both type 1 and 2 diabetes is an inadequate supply of insulin-producing tissue. In vitro transdifferentiation of human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) into insulin-producing cells could provide an abundant source of cells for this procedure. For this study, we isolated and characterized human UCB-MSCs and induced them in vitro to differentiate into islet-like cell clusters using a 15-day protocol based on a combination of high-glucose, retinoic acid, nicotinamide, epidermal growth factor, and exendin-4. These clusters appeared about 9 days after pancreatic differentiation; expressed pancreatic beta-cell markers, including insulin, glucagon, Glut-2, PDX1, Pax4, and Ngn3; and could synthesize and secrete functional islet proteins at the end of the inducing protocol. The insulin-positive cells accounted for (25.2-3.36)% of whole induced cells. Although insulin secretion of those insulin-producing cells did not respond to glucose challenge very well, human UCB-MSCs have the ability to differentiate into islet-like cells in vitro and may be a potential new source for islet transplantation.
机译：对于1型和2型糖尿病，成功进行胰岛移植的主要障碍是胰岛素生产组织的供应不足。人脐带血间充质干细胞（UCB-MSC）的体外转分化为产生胰岛素的细胞可以为该程序提供丰富的细胞来源。在这项研究中，我们分离并鉴定了人UCB-MSC，并在15天内根据高葡萄糖，视黄酸，烟酰胺，表皮生长因子和葡萄糖的组合，诱导它们分化为胰岛样细胞簇。 exendin-4。这些簇在胰腺分化后约9天出现。表达的胰岛β细胞标志物，包括胰岛素，胰高血糖素，Glut-2，PDX1，Pax4和Ngn3;并且可以在诱导方案结束时合成并分泌功能性胰岛蛋白。胰岛素阳性细胞占整个诱导细胞的（25.2-3.36）％。尽管那些产生胰岛素的细胞的胰岛素分泌不能很好地响应葡萄糖激发，但人UCB-MSC具有在体外分化为胰岛样细胞的能力，并且可能是胰岛移植的潜在新来源。

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