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首页> 外文期刊>Translational research: the journal of laboratory and clinical medicine >In vitro studies of amikacin-loaded human carrier erythrocytes.
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In vitro studies of amikacin-loaded human carrier erythrocytes.

机译：载有丁胺卡那霉素的人载体红细胞的体外研究。

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Erythrocyte-encapsulated antibiotics have the potential to provide an effective therapy against intracellular pathogens. The advantages over the administration of free antibiotics include a lower systemic dose, decreased toxicity, a sustained delivery of the antibiotic at higher concentrations to the intracellular site of pathogen replication, and increased efficacy. In this study, the encapsulation of amikacin by human carrier erythrocytes prepared using a hypo-osmotic dialysis was investigated. The effects of the initial amikacin dialysis concentration and hypo-osmotic dialysis time on the encapsulation efficiency of amikacin were determined, and the osmotic fragility and hematologic parameters of amikacin-loaded carrier erythrocytes were measured. The efficiency of amikacin entrapment by carrier erythrocytes was dependent on the initial dialysis concentration of the drug. Statistically significant differences in the osmotic fragility profiles between control and carrier erythrocytes were observed, which were dependent on the hypo-osmotic dialysis time and on the dialysis concentration of amikacin. Mean hematologic parameters were evaluated and compared with unloaded, native erythrocytes; the mean corpuscular volume (MCV) of amikacin-loaded carrier erythrocytes was statistically significant smaller. Amikacin demonstrated a sustained release from loaded erythrocytes over a 48-h period, which suggests a potential use of the erythrocyte as a slow systemic-release system for antibiotics.
机译：红细胞包裹的抗生素具有提供针对细胞内病原体的有效疗法的潜力。与施用游离抗生素相比，其优势包括较低的全身剂量，降低的毒性，将较高浓度的抗生素持续递送至病原体复制的细胞内部位以及提高的功效。在这项研究中，研究了使用低渗渗析制备的人载体红细胞对丁胺卡那霉素的包封。确定了初始丁胺卡那霉素透析浓度和低渗透析时间对丁胺卡那霉素包封效率的影响，并测定了加载丁胺卡那霉素的载体红细胞的渗透性和血液学参数。载体红细胞截留阿米卡星的效率取决于药物的初始透析浓度。观察到对照和载体红细胞在渗透性脆性曲线上的统计学显着差异，这取决于低渗透透析时间和阿米卡星的透析浓度。评估平均血液学参数，并与未加载的天然红细胞进行比较;阿米卡星载运的载体红细胞的平均红细胞体积（MCV）在统计学上较小。丁胺卡那霉素在48小时内可从负载的红细胞中持续释放，这表明红细胞有可能被用作抗生素的缓慢全身释放系统。

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