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首页> 外文期刊>Transactions of the American Ophthalmological Society. >Histological changes and wound healing response following noncontact holmium: YAG laser thermal keratoplasty.
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Histological changes and wound healing response following noncontact holmium: YAG laser thermal keratoplasty.

机译：非接触性following后的组织学变化和伤口愈合反应：YAG激光热角膜成形术。

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PURPOSE: To evaluate acute histological changes and the induced wound healing response in corneal tissue following noncontact holmium:YAG laser thermal keratoplasty (LTK). METHODS: LTK using 10 pulses and a range of radiant energies was performed on 3 human corneas one day prior ro their removal at penetrating keratoplasty. Rabbit corneas were treated with 10-pulse and 5-pulse LTK and followed for up to 3 months. Tissues were studies with light and transmission electron microscopy and immunohistochemistry. RESULTS: The amount of acute tissue injury increased with increasing pulse radiant energy. In human corneas, changes in the irradiated zones included epithelial cell injury and death loss of fine filamentous structure in Bowman's layer, disruption of stromal lamallae, and keratocyte injury and death. In the rabbit corneas, similar acute changes were noted. By 3 weeks, epithelial hyperplasia and stromal contraction were present. Wound healing in the rabbit corneas included repair of the epithelial attachment complex, keratocyte activation, synthesis of type I collagen, partial restoration of stromal keratan sulfate and type VI collagen, and retrocorneal membrane formation. Compared to 10-pulse treatments, 5-pulse treatments produced less acute tissue injury and had more rapid restoration of normal stromal architecture. CONCLUSION: Noncontact LTK produces acute epithelial and stromal tissue changes and in rabbit corneas stimulates a brisk wound healing response. These changes could contribute to postoperative regression of induced refractive correction. Further work is required to determine if reductions in the magnitude of acute tissue injury and induced wound healing response will enhance the efficacy and stability of LTK.
机译：目的：评价非接触性:： YAG激光热角膜成形术（LTK）后角膜组织的急性组织学变化和诱导的伤口愈合反应。方法：一天前在3个人类角膜上进行穿透性角膜移植手术，使用10个脉冲和一定范围的辐射能量进行LTK手术。兔角膜用10脉冲和5脉冲LTK治疗，随访3个月。用光电子显微镜和透射电子显微镜以及免疫组织化学研究组织。结果：急性组织损伤的程度随着脉冲辐射能的增加而增加。在人类角膜中，照射区的变化包括上皮细胞损伤和鲍曼层细丝状结构的死亡，死于基质层，角膜细胞损伤和死亡。在兔角膜中，注意到类似的急性变化。到3周时，出现上皮增生和间质收缩。兔角膜的伤口愈合包括上皮附着复合物的修复，角膜细胞活化，I型胶原蛋白的合成，硫酸角质素基质和VI型胶原蛋白的部分修复以及角膜后膜的形成。与10脉冲治疗相比，5脉冲治疗产生的急性组织损伤更少，并且恢复的正常基质结构更快。结论：非接触性LTK产生急性上皮和基质组织改变，并且在兔角膜中刺激轻快的伤口愈合反应。这些改变可能有助于术后屈光矫正的回归。需要进一步的工作来确定急性组织损伤和诱导的伤口愈合反应程度的降低是否会增强LTK的疗效和稳定性。

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