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首页> 外文期刊>Transplant immunology >TRAV and TRBV repertoire, clonality and the proliferative history of umbilical cord blood T-cells.
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TRAV and TRBV repertoire, clonality and the proliferative history of umbilical cord blood T-cells.

机译：TRAV和TRBV的库，克隆性和脐带血T细胞的增殖史。

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Umbilical cord blood (CB) has been used successfully as a source of hematopoietic stem cells for transplantation. But the distribution and clonality of T-cell receptor alpha variable region (TRAV) and T-cell receptor beta variable region (TRBV) subfamily T-cells, the naive T-cells level and the diversity of thymic recent output function in CB has not been yet clearly defined. In order to characterize the repertoire of CB T-cells, the CDR3 of 29 TRAV and 24 TRBV subfamily genes were analyzed in T-cells from 12 cord blood samples, using RT-PCR and genescan technique. To determine the proliferative history of CB T-cells, quantitative analysis of deltaRec-psiJalpha signal joint T-cell receptor excision circles (sjTRECs) was performed in mononuclear cells, CD3+, CD4+ and CD8+ T-cells from 20 CB samples by real-time PCR. In addition the analysis of 23 TRBV-TRBD1 sjTRECs in 10 cases of CB CD4+ T-cells and CB CD8+ T-cells was performed by semi-nested PCR. We found a marked restriction of TRBV expression pattern in CBMCs compared to peripheral blood mononuclear cells (PBMC), which expressed all 24 TRBV genes. All PCR products of TRAV and TRBV subfamilies from CB, except for 3 cases, displayed polyclonal rearrangement pattern. The deltaRec-psiJalpha sjTRECs counts were significantly higher in CB, than in PB samples. Also the number of detectable TRBV sjTRECs was higher in CB than in peripheral blood. In conclusion, our results indicate polyclonal but restricted repertoire and a very short proliferative history of CB T-cells. The incomplete repertoire and naivety of CB T-cells might be the reason that CB hematopoietic stem cells transplant recipients are less likely to develop graft vs host disease.
机译：脐带血（CB）已成功用作造血干细胞移植的来源。但是，在CB中，T细胞受体α可变区（TRAV）和T细胞受体β可变区（TRBV）亚家族T细胞的分布和克隆性，幼稚T细胞水平和胸腺近期输出功能的多样性尚无。尚未明确定义。为了表征CB T细胞库，使用RT-PCR和genescan技术在来自12个脐带血样品的T细胞中分析了29个TRAV和24个TRBV亚家族基因的CDR3。为了确定CB T细胞的增殖史，实时定量分析了20个CB样品中单核细胞，CD3 +，CD4 +和CD8 + T细胞中deltaRec-psiJalpha信号联合T细胞受体切除环（sjTRECs）的数量。 PCR。此外，通过半巢式PCR对10例CB CD4 + T细胞和CB CD8 + T细胞中的23个TRBV-TRBD1 sjTRECs进行了分析。我们发现与外周血单个核细胞（PBMC）相比，CBMC中TRBV表达模式受到明显限制，后者表达了所有24个TRBV基因。除3例外，来自CB的TRAV和TRBV亚家族的所有PCR产物均显示多克隆重排模式。 CB中的deltaRec-psiJalpha sjTRECs计数显着高于PB样品。此外，CB中可检测到的TRBV sjTRECs的数量高于外周血。总而言之，我们的结果表明多克隆但受限的库，并且CB T细胞的增殖史非常短。 CB T细胞库的不完整和幼稚可能是CB造血干细胞移植受者发生移植物抗宿主病的可能性较小的原因。

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