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首页> 外文期刊>Transplant immunology >The type of ATG matters - Natural killer cells are influenced differentially by Thymoglobulin, Lymphoglobulin and ATG-Fresenius.
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The type of ATG matters - Natural killer cells are influenced differentially by Thymoglobulin, Lymphoglobulin and ATG-Fresenius.

机译：ATG的类型很重要-天然杀伤细胞受到胸腺球蛋白，淋巴球蛋白和ATG-费森尤斯的影响。

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摘要

Although ATG is frequently used in hematopoietic stem cell transplantation and solid organ transplantation, little is known on its effects on NK cells, which mediate important functions in post-transplantation immunology. We incubated peripheral blood lymphocytes of healthy donors with Thymoglobulin, Lymphoglobulin or ATG-Fresenius. Cell death and apoptosis of NK cells and T cells were determined by flow cytometry using propidium iodide and Annexin V. As expected, there were no significant differences between the different ATGs regarding their T cell toxicity. Surprisingly, we found profound differences between the different ATGs regarding their impact on NK cells: In clinically relevant concentrations Lymphoglobulin had less toxic effects on NK cells as compared to Thymoglobulin or ATG-Fresenius: the median percentages of apoptotic or necrotic NK cells in response to 1 mug/ml Lymphoglobulin, ATG-Fresenius and Thymoglobulin were 2%, 35% and 38%, respectively (p<0.001). This is the first report of differential effects of different ATGs on NK cells. Lymphoglobulin appears to be superior to Thymoglobulin or ATG Fresenius regarding the preservation of NK cell mediated immunity. Randomized trials addressing the impact of different ATGs on lymphocyte subpopulations in the clinical setting are urgently warranted.
机译：尽管ATG常用于造血干细胞移植和实体器官移植，但对它对NK细胞的作用所知甚少，后者在移植后免疫学中起重要作用。我们将健康供体的外周血淋巴细胞与胸腺球蛋白，淋巴球蛋白或ATG-费森尤斯一起孵育。通过使用碘化丙啶和膜联蛋白V的流式细胞术确定NK细胞和T细胞的细胞死亡和凋亡。正如预期的那样，不同的ATG在T细胞毒性方面没有显着差异。出乎意料的是，我们发现不同的ATG在其对NK细胞的影响方面存在深远的差异：在临床上相关浓度下，与甲状腺球蛋白或ATG-费森尤斯相比，淋巴球蛋白对NK细胞的毒性作用较小：凋亡或坏死的NK细胞对1杯/毫升的淋巴球蛋白，ATG-费森尤斯和胸腺球蛋白分别为2％，35％和38％（p <0.001）。这是不同ATG对NK细胞的差异作用的第一个报道。就NK细胞介导的免疫的保存而言，淋巴球蛋白似乎优于胸球蛋白或ATG费森尤斯。迫切需要针对临床环境中不同ATG对淋巴细胞亚群的影响进行随机试验。

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